HPLC法测定蒙成药査格得日中羟基红花黄色素A的含量  

Content determination of safflomin A in Chagederi by HPLC

作　　者：孔德娟 王艳楠 王燕燕 任娅婷 KONG De-juan;WANG Yan-nan;WANG Yan-yan;REN Ya-ting(Institute for food and drug control of Tong Liao,028000 China)

机构地区：[1]通辽市食品药品检验所,内蒙古通辽028000

出　　处：《中国民族医药杂志》2021年第7期49-51,共3页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古自治区蒙医药标准化项目(2019-[MB022])。

摘　　要：目的:建立测定查格得日中羟基红花黄色素A含量的方法。方法:采用高效液相色谱法,色谱柱为InertSustainAQ C_(18)柱(250 mm×4.60 mm,5μm),流动相为甲醇-乙腈-0.7%磷酸溶液(含1.5%三乙胺)(26∶2∶72),流速1.0 mL/min,检测波长403 nm,柱温40℃。结果:羟基红花黄色素A在0.0285966~0.571932μg范围内呈良好线性关系,r^(2)=0.9999;羟基红花黄色素A平均加样回收率为100.7%,RSD=1.6%。结论:本方法操作简便,可准确测定査格得日中羟基红花黄色素A的含量。Objective:To establish a method to determine contents of safflomin A in Chagederi. Methods:Contents of safflomin A was determined by HPLC,the column was InertSustainAQ C_(18)(250 mm ×4.60 mm,5 μm),the mobile phase consisted of methanol-acetonitrile-0.7% phosphoric acid(26 ∶2 ∶72)(1.5% triethylamine),the flow rate was 1.0 m L/min,the detection wavelength was set at 403 nm,the column temperature was maintained at 40 ℃. Results:The linear range of safflomin A was 0.0285966 ~0.5719319 μg(r^(2)=0.9999). The mean recovery of safflomin A was 100.7%,RSD =1.6%. Conclusion:The method is accurate in operation,which can be used to determine contents of safflomin A in Chagederi.

关 键 词：查格得日 高效液相色谱法 羟基红花黄色素A 

分 类 号：R291.2[医药卫生—民族医学]