载CXCL12抗体靶向超声微泡制备及其体外卵巢癌细胞粘附性实验  

Preparation of CXCL12 antibody-targeted ultrasound microvesicle and its adhesion to ovarian cancer cells in vitro

作　　者：付丽君 向红[1] 曾倩倩[1] 胡蓉[1] FU Lijun;XIANG Hong;ZENG Qianqian;HU Rong(Department of Obstetrics and Gynecology Ultrasound,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院妇产超声科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2021年第8期892-896,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81660288);新疆维吾尔自治区自然科学基金(2016D01C267)。

摘　　要：目的制备携带基质细胞衍生因子1(CXCL12)抗体的靶向超声微泡,检测其一般物理特性及体外寻靶能力。方法通过生物素-亲和素桥接法将CXCL12抗体连接到声诺维微泡上,制备成靶向超声微泡,作为实验组,用流式细胞仪和荧光显微镜检测微泡的结合率及稳定性,体外粘附性实验观察靶向超声微泡与卵巢癌细胞的结合能力。以未连接CXCL12抗体的声诺维微泡作为对照组。结果实验组微泡的粒径均一,稳定性好,荧光显微镜下靶向微泡表面呈环状红色荧光,流式细胞仪靶向微泡结合率明显高于对照组(P<0.05),振荡后结合率略降低,但差异无统计学意义(P>0.05)。体外寻靶实验显示癌细胞周边呈花环状粘附,但对照组未见粘附,差异有统计学意义(P<0.05)。结论通过使用生物素-亲和素桥接法,可以让携带CXCL12抗体靶向超声微泡具有较高的结合率,并且具有较好的稳定性,在体外可与人卵巢癌SKOV3细胞牢固结合。Objective To prepare targeted ultrasound microvesicle carrying stromal cell derived factor 1(CXCL12)antibody,and to detect its general physical properties and target seeking ability in vitro.Methods CXCL12 antibody was connected to sonovir microvesicles by biotin-avidin bridging method,and targeted ultrasonic microvesicles were pre‐pared.As the experimental group,the binding rate and stability of the microvesicles were detected by flow cytometry and fluorescence microscopy.In vitro adhesion assay was conducted to observe the binding ability of targeted ultrasound microvesicles to ovarian cancer cells,and sonic novi microvesicles unattached to CXCL12 antibody were used as control group.Results The microvesicles in the experimental group showed uniform particle size and good stability.The surface of the targeted microvesicles showed ring red fluorescence under fluorescence microscope.The binding rate of the targeted microvesicles by flow cytometry was significantly higher than that of the control group(P<0.05),and the binding rate decreased slightly after oscillation,but the difference was not statistically significant(P>0.05).In vitro target search experiment showed that there was floral ring adhesion around the cancer cells,but no adhesion was observed in the control group,the difference was statistically significant(P<0.05).Conclusion By using biotin-avidin bridging method,targeted ultrasound microvesicles carrying CXCL12 antibody can have a high binding rate and good stability,and can firmly bind to human ovarian cancer SKOV3 cells in vitro.

关 键 词：CXCL12 靶向超声微泡 卵巢癌 体外寻靶 

分 类 号：R445.1[医药卫生—影像医学与核医学]