牡丹响应高温胁迫的转录组分析及PsHSP基因表达  

Transcriptome analysis and PsHSP gene expression of Paeonia suffruticosa in response to high temperature stress

作　　者：郝力慧 董彬 朱绍华[1,2,3] 马进 HAO Lihui;DONG Bin;ZHU Shaohua;MA Jin(Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;College of Landscape Architecture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学浙江省园林植物种质创新与利用重点实验室,浙江杭州311300 [2]浙江农林大学南方园林植物种质创新与利用国家林业和草原局重点实验室,浙江杭州311300 [3]浙江农林大学风景园林与建筑学院,浙江杭州311300

出　　处：《浙江农林大学学报》2021年第4期802-811,共10页Journal of Zhejiang A&F University

基　　金：浙江农林大学科研发展基金资助项目(2017FR016);元成环境股份企业研发项目(201801)。

摘　　要：【目的】夏季高温可导致牡丹Paeonia suffruticosa生长受限,花期缩短,观赏品质降低。研究牡丹耐热基因对牡丹热胁迫的分子机制。【方法】以牡丹品种‘羽红’‘Yuhong’为材料,对高温(40℃)和对照(25℃)处理后的叶片进行转录组测序,分析响应高温表达的差异基因;同时利用实时荧光定量PCR(qRT-PCR)验证差异基因,并对热激蛋白基因HSPs进行时空表达分析。【结果】测序共获得45.97 Gb数据,与对照组(25℃)相比,高温处理后4220个基因上调表达,3453个基因下调表达。通过基因本体(GO)分析发现,这些差异基因主要富集于代谢等生物学过程、细胞和膜结构等细胞成分以及结合和催化活性等分子功能条目;另外,通过全基因组及代谢途径数据库(KEGG)分析发现,碳代谢途径的差异基因数量最多。对牡丹热激蛋白PsHSP基因定量分析发现,PsHSP基因随着高温处理时间呈上升表达趋势,24 h达到最高,之后便呈现下降趋势。【结论】高温显著影响牡丹的代谢和生物合成等过程,进而影响牡丹的生长和发育。PsHSP基因可在短期内迅速响应高温胁迫并参与牡丹的耐热调控。图4表6参34。[Objective]High temperature in summer can lead to growth restriction,shorter flowering period and lower ornamental quality of Paeonia suffruticosa.This study aims to explore the molecular mechanism of heat stress induced by heat tolerance genes in P.suffruticosa.[Method]Using P.suffruticosa‘Yuhong’as material,transcriptome sequencing of leaves treated with high temperature(40℃)and room temperature(25℃)was conducted to analyze the differentially expressed genes in response to high temperature.Meanwhile,the differential genes were verified by real-time fluorescence quantitative PCR(qRT-PCR),and the spatio-temporal expression of heat shock protein gene HSPs was analyzed.[Result]A total of 45.97 Gb data were obtained by sequencing.Compared with the control group(25℃),4220 genes were up-regulated and 3453 genes were down-regulated after high temperature treatment.Through gene ontology(GO)analysis,it was found that these differential genes were mainly concentrated in biological processes such as metabolism,cellular components such as cell and membrane structure,and molecular functional items such as binding and catalytic activity.In addition,genome-wide and metabolic pathway database(KEGG)analysis showed that the number of differential genes in carbon metabolic pathway was the largest.Quantitative analysis of heat shock protein Ps HSP gene showed that the expression of Ps HSP gene increased with the time of high temperature treatment,reached the peak at 24 h,and then showed a downward trend.[Conclusion]High temperature significantly affects the metabolism and synthesis of P.suffruticosa,and further affects its growth and development.Ps HSP gene can rapidly respond to high temperature stress in a short time and participate in heat tolerance regulation in P.suffruticosa.[Ch,4 fig.6 tab.34 ref.]

关 键 词：植物学 牡丹 高温胁迫 转录组 热激蛋白 

分 类 号：Q943[生物学—植物学]