蜘蛛抱蛋(Aspidistra elatior)炭疽病病原菌的分离与鉴定  

Isolation and identification of pathogen causing anthracnose on Aspidistra elatior

作　　者：黄钰婷 孙洁 张慧娟[1] 朱晏 蒋明[1] HUANG Yuting;SUN Jie;ZHANG Huijuan;ZHU Yan;JIANG Ming(College of Life Sciences, Taizhou University, Taizhou 318000, China)

机构地区：[1]台州学院生命科学学院,浙江台州318000

出　　处：《浙江农业学报》2021年第8期1461-1467,共7页Acta Agriculturae Zhejiangensis

基　　金：台州市大学生科技创新项目(2019年度)。

摘　　要：为明确蜘蛛抱蛋(Aspidistra elatior)炭疽病病原菌的种类,本研究对采集的炭疽病样本进行分离纯化培养,得到1株蜘蛛抱蛋炭疽病病原真菌YYL,采用针刺回接实验测定其致病性,并在形态学鉴定基础上,克隆其ITS、ACT、GAPDH和CAL片段进行多基因联合鉴定。结果表明,该病原菌具有较强致病性,其菌落、菌丝和分生孢子形态符合百合炭疽菌(Colletotrichum lilii)的特征,且与百合炭疽菌相似度最高,其ITS和ACT序列与百合炭疽菌完全一致。综合形态学特征和分子生物学鉴定结果,确定引起蜘蛛抱蛋炭疽病的病原菌为百合炭疽菌。To identify the pathogenic fungus causing anthracnose on Aspidistra elatior,tissue isolation method was applied on the samples collected and a fungus strain YYL was obtained.Acupuncture method was used to confirm its pathogenicity.Based on the morphological identification results,ITS,ACT,GAPDH,and CAL fragments were cloned for multigene analysis.The results showed that YYL had high pathogenicity,and the characteristics of colony,hyphae,and conidium were well matched to Colletotrichum lilii.The gene fragments from YYL shared the highest similarity with C.lilii,and the ITS and ACT sequences of both YYL and C.lilii were identical.By combining the morphological features and molecular biological identification results,C.lilii was identified as the pathogen causing anthracnose on Aspidistra elatior.

关 键 词：蜘蛛抱蛋 炭疽病 病原菌 百合炭疽菌 

分 类 号：S432.1[农业科学—植物病理学]