TRAIL基因多态性及其血清水平与急性白血病的相关性研究  

STUDY ON THE RELATIONSHIP BETWEEN THE LEVELS OF TRAIL POLYMORPHISM,SERUM LEVELS AND ACUTE LEUKEMIA

作　　者：闫晓华 刘瑞磊[2] 彭宝相 张纪云 孙运芳 程振娜 YAN Xiao-hua;LIU Rui-lei;PENG Bao-xiang;ZHANG Ji-yun;SUN Yun-fang;CHENG Zhen-na(Shandong Medical College,Linyi 276000,China)

机构地区：[1]山东医学高等专科学校,山东临沂276000 [2]临沂市人民医院 [3]临沂市肿瘤医院

出　　处：《山东医学高等专科学校学报》2021年第4期241-244,共4页Journal of Shandong Medical College

基　　金：山东省医药卫生科技发展计划项目(No.2017WS834);山东省高等学校科技计划项目(No.J17KA239)。

摘　　要：目的探讨TRAIL与急性白血病的关联性。方法收集85例AL患者,同期选择体检者80例为对照组。采用测序法及PCR-RFLP法分析两组TRAIL基因第五外显子3'-UTR区1525G/A、1588G/A、1595C/T多态性;采用ELISA检测血清sTRAIL浓度。结果AL组与对照组TRAIL基因1525(G/A)、1588(G/A)、1595(C/T)基因型及等位基因分布不一致(χ^(2)=6.504,6.739;P<0.05);AL组血清sTRAIL浓度明显高于对照组(t=2.86,P<0.01)。结论TRAIL基因(1525G/A、1588G/A及1595C/T)多态性及血清sTRAIL水平与AL的发生有相关性。Objective To explore the relationship between TRAIL and acute leukemia.Methods A total of 85 patients with AL were collected.At the same time,80 patients were selected as the control group.Sequencing and PCR-RFLP methods were used to analyze the 1525G/A,1588G/A,and 1595C/T polymorphisms in the fifth exon 3'-UTR region of the two groups of TRAIL genes.The serum sTRAIL concentration was detected by ELISA.Results In the AL group and the control group,TRAIL gene 1525(G/A),1588(G/A),1595(C/T)genotypes and allele distributions were inconsistent(χ^(2)=6.504,6.739;P<0.05).In,AL group,the serum sTRAIL concentration was significantly higher than that of the control group(t=2.86,P<0.01).Conclusion TRAIL gene(1525G/A,1588G/A and 1595C/T)polymorphisms and serum sTRAIL levels are correlated with the occurrence of AL.

关 键 词：急性白血病 肿瘤坏死因子相关凋亡诱导配体 多态性 

分 类 号：R557[医药卫生—血液循环系统疾病]