体外培养细胞中细胞器的分离与纯化  

Isolation and Purification of Organelles from Cultured Cells

作　　者：周梦 史熊杰 ZHOU Meng;SHI Xiongjie(School of Life Sciences,Wuhan University,Hubei Wuhan 430072)

机构地区：[1]武汉大学生命科学学院,湖北武汉430072

出　　处：《生物化工》2021年第4期130-134,共5页Biological Chemical Engineering

摘　　要：在现代细胞生物学研究中,为了揭示生物大分子空间定位、相互关系及其功能,高效快速地从体外培养的细胞中分离纯化出纯度较高的细胞器就显得至关重要。本综述主要介绍两种常见的分离纯化方式——离心分离和亲和免疫纯化分离,以及在细胞器分离纯化过程中,细胞破碎方法和溶剂的选择,两种方法的优缺点和纯化后的鉴定方法。In modern cell biology research,in order to reveal the spatial localization,interrelationship and function of biomacromolecules,it is very important to efficiently isolate and purify organelles with high purity from cultured cells in vitro.This review mainly introduces two common separation and purification methods:centrifugal separation and affinity immunopurification separation,as well as the selection of cell crushing methods and solvents in the process of organelle separation and purification,the advantages and disadvantages of the two methods and the identification methods after purification.

关 键 词：细胞器纯化 细胞破碎 离心分离 亲和免疫纯化 鉴定 

分 类 号：Q2-33[生物学—细胞生物学]