GLIS1基因多态性与绵羊产羔数之间的关系研究  

GLIS1 gene polymorphisms and their association with litter size in sheep

作　　者：周梅[1,2] 刘秋月 狄冉[1] 胡文萍[1] 王翔宇[1] 张效生[3] 张金龙[3] 储明星[1] ZHOU Mei;LIU Qiuyue;DI Ran;HU Wenping;WANG Xiangyu;ZHANG Xiaosheng;ZHANG Jinlong;CHU Mingxing(Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences,Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering,Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Hefei 230031,China;Tianjin Institute of Animal Sciences,Tianjin 300381,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,农业农村部动物遗传育种与繁殖重点实验室,北京100193 [2]安徽省农业科学院畜牧兽医研究所,猪分子数量遗传学安徽省农业科学院重点实验室,畜禽产品安全工程安徽省重点实验室,合肥230001 [3]天津市畜牧兽医研究所,天津300381

出　　处：《上海农业学报》2021年第4期103-107,共5页Acta Agriculturae Shanghai

基　　金：国家转基因科技重大专项(2016ZX08009-003-006和2016ZX08010-005-003);国家自然科学基金项目(31772580);国家肉羊产业技术体系专项(CARS-38);中国农业科学院科技创新工程(ASTIP-IAS13);农业科研杰出人才及其创新团队项目[农办人(2015)62号];国家万人计划科技创新领军人才项目(W02020274);天津市农业科技成果转化与推广项目(201704020);安徽省农业科学院博士启动基金。

摘　　要：为了探究GLIS1基因多态性与绵羊产羔数之间的关系,利用Sequenom MassARRAY^(®)SNP技术对绵羊GLIS1基因的2个SNP位点多态性进行检测,并与绵羊产羔数进行关联分析。结果表明:在单羔和多羔绵羊品种之间,GLIS1基因2个SNP位点基因型和等位基因频率有极显著差异(P<0.01);关联分析显示,g.27775611 T>C位点与小尾寒羊第二胎产羔数显著相关(P<0.05),而g.27857114 T>G位点与各胎产羔数均无显著关联(P>0.05)。研究初步表明,g.27775611 T>C位点可能参与绵羊产羔数性状的调控,而g.27857114 T>G位点可能不是影响绵羊产羔数的关键位点。该研究可为绵羊高繁殖力基因及关键位点的筛选提供参考。To investigate the association between polymorphisms of GLIS1 and litter size in sheep,Sequenom MassARRAY^(®)SNP assay was applied to genotype two SNPs of sheep GLIS1 gene,and the correlation between GLIS1 and litter size in sheep was analyzed.The results showed that extremely significant differences were found in the genotypes and allele frequencies of the two SNP loci of GLISl gene between uniparous and multiparous sheep breeds.(P<0.01).Association analysis showed that polymorphism of g.27775611 T>C was significantly correlated with the litter size of the second parity of Small Tail Han sheep(P<0.05),while g.27857114 T>G locus had no significant correlation with the litter size of Small Tail Han sheep(P>0.05).The study preliminarily showed that the g.27775611 T>C locus of GLISl gene may be involved in the regulation of litter size trait in sheep,while the g.27857114 T>G locus may not be a key site affecting the litter size of sheep.This study can provide a reference for screening the genes and key loci of high fertility sheep.

关 键 词：绵羊 GLIS1基因 SNP分型 产羔数 

分 类 号：S826[农业科学—畜牧学]