四川猪传染性胸膜肺炎放线杆菌耐药基因流行规律与表型相关性研究  

Study on the Epidemiology Regularity and Phenotype Correlation of Drug-resistant Genes of Infectious Actinobacillus pleuropneumoniae in Sichuan

作　　者：王佳凡 郑博文 杨超 马晓平 曹三杰 黄小波 WANG Jiafan;ZHENG Bowen;YANG Chao;MA Xiaoping;CAO Sanjie;HUANG Xiaobo(Research Center of Swine Disease,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Department of Biological Engineering,Sichuan Water Conservancy Vocational College,Chengdu 611231,China)

机构地区：[1]四川农业大学动物医学院猪病研究中心,四川成都611130 [2]四川水利职业技术学院生物工程系,四川成都611231

出　　处：《云南农业大学学报（自然科学版）》2021年第4期631-639,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：四川省科技厅基础研究计划项目(2018JY0183)。

摘　　要：【目的】掌握四川省部分规模化养猪场猪传染性胸膜肺炎放线杆菌耐药基因流行规律与耐药表型相关性。【方法】对疑似患有猪传染性胸膜肺炎的病猪肺脏组织进行细菌分离鉴定和生化试验,并对分离株进行系统进化分析、耐药表型和耐药基因的检测。【结果】从病变肺脏组织分离到11株疑似猪传染性胸膜肺炎放线杆菌菌株,呈革兰氏阴性短杆状,生化试验和DNA分子生物学鉴定11株分离株为猪传染性胸膜肺炎放线杆菌。11株分离株呈多重耐药现象,对四环素和多西环素耐药率为90.90%;对盐酸林可霉素、复方新诺明和链霉素耐药率为54.55%;对盐酸克林霉素、庆大霉素、氯霉素和氟苯尼考耐药率为27.27%;对卡那霉素和红霉素耐药率分别为18.18%和9.10%。检测到sul3、tetB、tetC、ermB、floR、ant(6')-Ia、aph(2'')-Id和aac(6')-Ie-aph(2'')-Ia 8种耐药基因,携带率分别为100%、40.0%、100%、100%、100%、85.7%、14.3%和14.3%,与耐药表型相符。【结论】该地区规模化养猪场发生呼吸道感染死亡育肥猪细菌性病原是猪传染性胸膜肺炎放线杆菌,表现为多重耐药;分离株携带较多的耐药基因且与表型一致,为该病的防控和临床用药提供了科学依据。[Purpose]In order to understand the relationship between the epidemic regularity of resistance genes and drug resistance phenotype of Actinobacillus pleuropneumoniae in some large pig farms in Sichuan Province.[Method]Bacterial isolation,identification and biochemical tests were performed on the lung tissues of diseased pigs suspected of having porcine infectious pleuropneumonia and the isolates were subjected to systematic evolution analysis,drug resistance phenotype and drug-resistance gene detection.[Result]11 suspected isolates of A.pleuropneumoniae were isol-ated from the diseased lung tissues,which were Gram-negative short rods.Biochemical tests and DNA molecular biology identified 11 isolates as A.pleuropneumoniae.The 11 isolates showed mul-tiple drug resistance,among which the drug resistance rate of tetracycline and doxycycline was 90.90%.The drug resistance rate of lincomycin,cotrimoxazole and streptomycin was 54.55%.The drug resistance rate of clindamycin,gentamicin,chloramphenicol and florfenicol was 27.27%.The drug resistance rates to kanamycin and erythromycin were 18.18%and 9.10%,respectively.Detected 8 drug resistance genes,sul3,tetB,tetC,ermB,floR,ant(6')-Ia,aph(2'')-Id and aac(6')-Ie-aph(2'')-Ia,and the carrying rates were 100%,40.0%,100%,100%,85.7%,14.3%and 14.3%,respectively,which were consistent with the drug-sensitive phenotype.[Conclusion]This study shows that the bacterial pathogen of fattening pigs from respiratory tract infections in large-scale pig farms in this area is A.pleuropneumoniae,which exhibits multi-drug resistance,and isolates carried more drug res-istance genes and were consistent with the phenotype,which provided the basis for the prevention and control of this disease and clinical medication.

关 键 词：猪传染性胸膜肺炎放线杆菌 分离鉴定 耐药表型 耐药基因 

分 类 号：S858.28[农业科学—临床兽医学]