TetR家族转录调控因子配体的研究进展  

Ligands of TetR family transcriptional regulators: a review

作　　者：吴攀攀 李博文[1] 陈克涛 吴杭 张部昌 Panpan Wu;Bowen Li;Ketao Chen;Hang Wu;Buchang Zhang(Institutes of Physical Science and Information Technology,Anhui University,Hefei 230601,Anhui,China;College of Chemistry and Chemical Engineering,Anhui University,Hefei 230601,Anhui,China;School of Life Sciences,Anhui University,Hefei 230601 Anhui,China)

机构地区：[1]安徽大学物质科学与信息技术研究院,安徽合肥230601 [2]安徽大学化学与化工学院,安徽合肥230601 [3]安徽大学生命科学学院,安徽合肥230601

出　　处：《生物工程学报》2021年第7期2379-2392,共14页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.31972930,31570074);安徽高校协同创新项目(No.GXXT-2019-035)资助。

摘　　要：TetR家族转录调控因子(TetR family transcriptional regulators,TFRs)广泛分布于细菌与古菌中,最早被发现的是可控制大肠杆菌四环素外排泵的TetR。TFRs拥有DNA结合和多样的配体感知能力,其作用机制复杂。配体小分子诱导TFRs产生构象变化,抑制或促进TFRs对其靶点的控制。目前已知的TFRs配体种类繁多,包括糖类、蛋白质、脂肪酸及其衍生物、金属离子等。配体的多样性使得TFRs调节范围很广,包括从基础碳代谢、氮代谢到群体感应、抗生素生物合成等一系列生理过程。文中主要从介导调控角度介绍TFRs配体的研究进展,并结合笔者实验室的研究工作,重点阐述TFRs配体在基础碳代谢、脂肪酸生物合成与降解等初级和次级代谢产物合成过程中的作用机制,及其在基因线路开发和抗生素合成基因的激活等应用方面的研究进展。TetR family transcriptional regulators(TFRs) are widely distributed in bacteria and archaea, and the first discovered TFR was confirmed to control the expression of tetracycline efflux pump in Escherichia coli. TFRs can bind DNAs and ligands. Small molecule ligands can induce conformational changes of TFRs, inhibiting or promoting TFRs to control target gene expression. Currently, TFRs have a wide variety of ligands, including carbohydrates, proteins, fatty acids and their derivatives, metal ions, and so on. Due to the diversity of ligands, TFRs regulate a wide range of physiological processes, from basic carbon metabolism and nitrogen metabolism to quorum sensing and antibiotic biosynthesis. On the basis of the recent studies in our laboratory and the literature, we review here the regulatory mechanism mediated by ligands of TFRs in primary and secondary metabolism, as well as the application of ligands for TFRs in the development of gene route and the activation of antibiotic biosynthesis.

关 键 词：细菌 古菌 TetR家族转录因子 配体 反馈调控 前馈控制 

分 类 号：Q75[生物学—分子生物学]