实时无标记细胞分析系统(RTCA)在药物心肌毒性检测和生物活性研究中的应用  

Evaluation of drug myocardial toxicity and biological activity by real time xCELLigence analysis: a review

作　　者：张旭 王瑞莹 陈荣昌[2] 徐丽娇 孙晓波[2] 于淼 孙桂波[2] Xu Zhang;Ruiying Wang;Rongchang Chen;Lijiao Xu;Xiaobo Sun;Miao Yu;Guibo Sun(Engineering Research Center for Medicine,College of Pharmacy,Harbin University of Commerce,Harbin 150076,Heilongjiang,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100094,China)

机构地区：[1]哈尔滨商业大学药学院药物工程技术研究中心,黑龙江哈尔滨150076 [2]中国医学科学院北京协和医学院药用植物研究所,北京100094

出　　处：《生物工程学报》2021年第7期2425-2434,共10页Chinese Journal of Biotechnology

基　　金：中央级公益性科研院所基本科研业务费项目基金(No.2018PT35030);“重大新药创制”科技重大专项资金(No.2018ZX09711001-009);北京协和医科大学研究生创新基金(No.2019-1007-16)资助。

摘　　要：实时无标记细胞分析系统(Real timexCELLigence analysissystem,RTCA)是一种新型细胞检测技术,能够连续监测、记录及分析细胞活动产生的各种信息,在药物研究中的心肌毒性评估和细胞生物活性考察方面都可以发挥重要作用。文中首先对RTCA的原理与特点进行了介绍,然后分别对RTCA在心肌毒性和细胞生物活性研究中的应用现状进行了综述,为了解和使用RTCA提供了参考。RTCA技术具有实时无标记、非侵入性、高通量、准确性高等特点,不仅有助于药物研究和新药开发,在其他一些领域也有着广阔良好的应用前景。Realtime xCELLigence analysis(RTCA) is a new cell detection technology to continuously monitor, record and analyze a variety of information generated by cell activity. In drug research, it plays an important role in assessing myocardial toxicity and cell biological activity. Here, we first introduce the underlying mechanisms and characteristics of RTCA. Then we review the applications of RTCA in the research of myocardial toxicity and cell biological activity, to provides the fundamental baseline for understanding and exploiting RTCA. With the real-time, unlabeled, non-invasive, high throughput, and high accuracy features, RTCA not only promotes drug research and development, but also has a broad and good application prospect in other fields.

关 键 词：实时无标记细胞分析系统 药物研究 心肌毒性 生物活性 细胞检测 

分 类 号：R446[医药卫生—诊断学]