慢性鼻窦炎伴鼻息肉黏膜上皮紧密连接的表达研究  

Researches on the expression of tight junctions on chronic rhinosinusitis with nasal polyps

作　　者：段甦[1] 韩新玲 李颖[3] 张罗[1,2,3] DUAN Su;HAN Xinling;LI Ying;ZHANG Luo(Department of Allergy,Capital Medical University,Beijing,100730,China;Department of Otolaryngology Head and Neck Surgery,Key Laboratory of Otolaryngology Head and Neck Surgery(Capital Medical University),Ministry of Education,Beijing Tongren Hospital,Capital Medical University,Beijing,100730,China;Beijing Institute of Otolaryngology,Beijing Key Laboratory of Nasal diseases,Beijing,100005,China)

机构地区：[1]首都医科大学附属北京同仁医院鼻过敏科,北京100730 [2]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京100730 [3]北京市耳鼻咽喉科研究所,鼻病研究北京市重点实验室,北京100005

出　　处：《中国耳鼻咽喉头颈外科》2021年第7期440-443,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金重点项目(81630023);国家自然科学基金面上项目(81870697);国家重点研发计划(2016YFC0905200);长江学者与创新团队发展计划(IRT13082);北京市医院管理局青苗人才计划“使命计划”(SML20150203);医学与健康创新工程(2019-I2M-5-022)。

摘　　要：目的本研究旨在研究慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者中不同嗜酸性粒细胞(Eos)比例的鼻息肉黏膜上皮紧密连接(tight junctions,TJs)的表达情况。方法在首都医科大学附属北京同仁医院鼻过敏科手术患者中,收集5例正常对照鼻黏膜和17例CRSwNP鼻息肉组织,应用实时荧光定量PCR和免疫荧光共聚焦显微镜检测TJs mRNA和蛋白的表达情况。结果实时荧光定量PCR结果显示claudin-1、claudin-4、claudin-7、occludin、ZO-1和ZO-2 mRNA在CRSwNP组表达较正常对照组下调,并且呈现Eos越多,表达越少的趋势;免疫荧光共聚焦显微镜检测同样发现CRSwNP中claudin-1、claudin-4、claudin-7、occludin表达减弱与Eos浸润程度呈一定相关性。结论CRSwNP患者鼻息肉黏膜上皮细胞多种TJs表达下降,Eos浸润程度可能对TJs的表达下降具有一定的影响。OBJECTIVE To investigate the expression of tight junctions(TJs)on the epithelial cells of chronic rhinosinusitis with nasal polyps(CRSwNP)of different eosinophils(Eos)proportions.METHODSFive normal nasal mucosa samples and 17 CRSwNP mucosa samples of patients who had endonasal endoscopic surgery were enrolled.Real-time fluorescence quantitative PCR was conducted to evaluate the mRNA expression of claudin-1,claudin-4,claudin-7,occludin,ZO-1 and ZO-2 and immunofluorescence was performed to exam the expression of claudin-1,claudin-4,claudin-7,occludin.RESULTSThem RNA expressions of claudin-1,claudin-4,claudin-7,occludin,ZO-1 and ZO-2 were decreased in CRSwNP with the increasing of Eos proportions,and the immunofluorescence also demonstrated discontinuous and broken expression of claudin-1,claudin-4,claudin-7 and occludin in an Eos-related way.CONCLUSION Our data suggest that Eos proportions may influence epithelial barrier function in CRSwNP patients by regulating TJ protein expression.

关 键 词：鼻窦炎 鼻息肉 嗜酸细胞 紧密连接部 上皮屏障 

分 类 号：R765.9[医药卫生—耳鼻咽喉科]