子宫腺肌病患者内膜组织淋巴增强结合因子1、β-连环素表达及临床意义  

Expression and clinical significance of the levels of lymphoid enhancer-binding factor 1 andβ-catenin in endometrium tissue of women with adenomyosis

作　　者：黄先华 刘婷 王青 HUANG Xianhua;LIU Ting;WANG Qing(Yiling District Maternal and Child Health Care Hospital, Yichang, Hubei Province, 443000)

机构地区：[1]湖北省宜昌市夷陵区妇幼保健院,443000

出　　处：《中国计划生育学杂志》2021年第7期1444-1448,1542,共6页Chinese Journal of Family Planning

摘　　要：目的:检测子宫腺肌病患者在位、异位内膜组织中淋巴增强结合因子1(LEF1)、β-连环素(β-catenin)表达水平及其临床意义。方法:选取2019年1月-2020年3月因子宫腺肌病于本院就诊并行子宫切除术的患者89例,分为在位内膜组织组及异位内膜组织组;宫颈原位癌患者86例正常子宫内膜组织作为对照组。采用实时荧光定量PCR(qRT-PCR)法检测各内膜组织中LEF1 mRNA、β-catenin mRNA表达水平,免疫组织化学法检测内膜组织中LEF1、β-catenin蛋白表达;Pearson法分析子宫腺肌病内膜组织LEF1 mRNA与β-catenin mRNA相关性及与临床特征关系。结果:LEF1、β-catenin mRNA表达水平及蛋白阳性表达率均异位内膜组最高、在位内膜组居中、对照组最低(P<0.05)。子宫腺肌病在位、异位内膜组织LEF1 mRNA与β-catenin mRNA表达水平均呈正相关(r=0.475、0.658,P<0.05),在位、异位内膜组织中LEF1、β-catenin阳性表达与LEF1、β-catenin阴性表达者子宫大小/腺肌病灶比较有差异(P<0.05)。结论:LEF1、β-catenin在子宫腺肌病患者异位、在位内膜组织中表达水平升高,其异常表达可能与子宫腺肌病有关。Objective:To detect the expression levels and clinical significance of lymphoid enhancer-binding factor 1(LEF1)andβ-catenin in eutopic and ectopic endometrium tissues of women with adenomyosis.Methods:89 women with adenomyosis after hysterectomy were selected as the research objects from January 2019 to March 2020.The tissues of eutopic endometrium of the women were included in group A and the tissues of ectopic endometrium were included in group B,respectively.During the same time,the tissues of normal endometrium from 86 patients with cervical carcinoma were selected in group C.Real-time fluorescent quantitative PCR(qRT-PCR)was used to detect the expression levels of mRNA of LEF1 andβ-catenin,and immunohistochemistry was used to detect the expression levels of protein of LEF1 andβ-catenin protein.Pearson method was used to analyze the correlation between LEF1 mRNA level andβ-catenin mRNA level in the endometrium tissues of the women with adenomyosis,and the relationship between the levels of LEF1 mRNA andβ-catenin mRNA in the endometrium tissues of these women,and their clinical features.Results:The expression level of mRNA and positive rates of protein of LEF1 andβ-catenin in group B were the highest,followed by those in group A,and which in group C were lowest(P<0.05).The expression levels of LEF1 mRNA andβ-catenin mRNA in the eutopic and ectopic endometrium tissue of women with adenomyosis were positively correlated(r=0.475,0.658,P<0.05).There were significant difference in the uterine size and adenomyosis status between the women with positive expression of LEF1 andβ-catenin in the eutopic and ectopic endometrium tissue and the women with negative expression of LEF1 andβ-catenin in the eutopic and ectopic endometrium tissue(P<0.05).Conclusion:The expression levels of LEF1 andβ-catenin in the ectopic and eutopic endometrium of the women with adenomyosis increase,and their abnormal expression may be related to the pathogenesis of adenomyosis.

关 键 词：子宫腺肌病 异位内膜组织 在位内膜组织 淋巴增强结合因子1 Β-连环素 

分 类 号：R711.71[医药卫生—妇产科学]