自制吹干装置在滤纸干血片样本制备中的应用  

Application of self-made drying device in the preparation of dried blood filter paper samples

作　　者：谌玉佳 孙智勇 王少静 杨江涛 吴本清 CHEN Yujia;SUN Zhiyong;WANG Shaojing;YANG Jiangtao;WU Benqing(The First Affiliated Hospital of Jinan University,Guangzhou 510632,Guangdong,China;Shenzhen Aone Medical Laboratory,Shenzhen 518000,Guangdong,China;University of Chinese Academy of Sciences Shenzhen Hospital,Shenzhen 518000,Guangdong,China)

机构地区：[1]暨南大学第一临床医学院,广东广州510632 [2]深圳爱湾医学检验实验室,广东深圳518000 [3]中国科学院大学深圳医院,广东深圳518000

出　　处：《检验医学》2021年第8期857-863,共7页Laboratory Medicine

基　　金：深圳市科技创新委员会资助项目(JCYJ20180502163111336)。

摘　　要：目的探讨自制吹干装置在滤纸干血片样本制备中的应用价值。方法将50μL全血滴在直径为12 mm的滤纸片上,分别以自然晾干和自制吹干装置干燥2种方式干燥血片。记录不同实验条件下血片干燥状态随时间变化的情况。采用液相色谱-串联质谱(LC-MS/MS)检测滤纸干血片样本中8种氨基酸和15种酰基肉碱水平,比较不同打孔部位[中心及周边部位(左上、右上、左下、右下)]之间检测结果的差异和不同干燥方式之间检测结果的差异,同时评价精密度和准确性。结果在室温(23±2)℃、湿度60%±5%的条件下,自然晾干和采用吹干装置使血片达到完全干燥的时间分别为95和40 min;但在室温(23±2)℃、湿度80%±5%的条件下,2种干燥方式使血片达到完全干燥的时间均为115 min。2种干燥方式周边打孔与中央打孔取样的检测结果比较,除自然干燥方式中辛酰肉碱(C8)的差异为-15.51%、3-羟基棕榈烯酰肉碱(C16-OH)的差异为-19.23%、即刻用吹干装置干燥方式中C16-OH的差异为-16.67%外,其他项目的差异为-14.81%~14.61%。不同干燥方式下氨基酸和肉碱的变异系数(CV)为2.85%~21.98%、准确度为97.08%~118.18%,均符合要求。结论自制吹干装置可有效提高血片的干燥效率,且不会对氨基酸和酰基肉碱的检测结果造成影响,可用于滤纸干血片的制备。Objective To investigate the value of self-made drying device in the preparation of dried blood filter paper samples.Methods Totally,50μL of whole blood were dropped onto the filter papers to make blood spots(the diameter was 12 mm),and fresh blood spots were dried by natural air drying or by self-made drying device.The changes of drying state of blood spots with time under different experimental conditions were recorded.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was recruited to determine the contents of 8 kinds of amino acids and 15 kinds of carnitines in the dried blood spots.The results were compared between different punching parts[center and peripheral parts(upper left,upper right,lower left and lower right)]and between different drying methods,and the precision and accuracy were evaluated.Results At room temperature of(23±2)℃and humidity of(60±5)%,it took 95 and 40 min to dry the blood filter paper samples by natural air drying and by the self-made drying device,respectively.However,at room temperature of(23±2)℃and humidity of(80±5)%,it took 115 min for both methods to dry the blood filter paper samples completely.Except for octanoyl carnitine(C8)and 3-hydroxypalmitoyl carnitine(C16-OH)in the natural air drying with a difference of-15.51%and-19.23%,respectively,and C16-OH in the self-made drying device with a difference of-16.67%,the difference of results between peripheral and center punching sampling was-14.81%-14.61%.The coefficient of variation(CV)(2.85%-21.98%)and accuracy(97.08%-118.18%)of amino acid and carnitine by different drying methods meet the requirements.Conclusions The self-made drying device can improve the drying efficiency of blood filter paper,and it will not affect the results of amino acid and carnitine,suggesting that it can be used for the preparation of dried blood filter paper.

关 键 词：自制吹干装置 滤纸干血片 色谱效应 精密度 准确度 

分 类 号：R446.1[医药卫生—诊断学]