罗丹明B修饰的树枝状分子作为核酸递送载体的研究  

Rhodamine B Modified Dendrimers as Efficient Nucleic Acid Delivery Vectors

作　　者：高永光 贾静娴[1] 王丽红[1] 张红霞[1] 陈伟[1] Gao Yongguang;Jia Jingxian;Wang Lihong;Zhang Hongxia;Chen Wei(Department of Chemistry,Tangshan Normal University,Tangshan,063000)

机构地区：[1]唐山师范学院化学系,唐山市063000

出　　处：《化学通报》2021年第8期829-834,819,共7页Chemistry

基　　金：唐山师范学院科学研究基金项目(2021A03);河北省教育厅科技重点项目(ZD2021408);河北省自然科学基金项目(B2021105001);陕西省自然科学基金项目(2019JM-230)资助。

摘　　要：设计合成了三种罗丹明B修饰的树枝状分子核酸递送载体GR-1、GR-2和GR-3,并通过核磁共振氢谱分析了树枝状分子表面连接的罗丹明B的数目。琼脂糖凝胶电泳实验表明,树枝状分子表面的罗丹明B数目越少,对RNA的浓缩效果越好。在三种核酸递送材料中,GR-3对Antagomir-138-5p的递送效率最高,基因沉默效率70%左右,高于商业化的转染试剂Lipofectamine 2000。Three dendrimers(GR-1,GR-2 and GR-3)modified with rhodamine B were designed and synthesized.The number of rhodamine B conjugated to the periphery of dendrimers was determined using 1H NMR.The agarose gel electrophoresis showed that the miRNA retardation ability was obviously improved with the decreasing number of rhodamine B on the surface of PAMAM dendrimers.Among the three materials,GR-3 showed the best transfection efficacy in RNA-mediated gene delivery,and gene silence efficacy was up to 70%,which was higher than the commercial transfection agent Lipofectamine 2000.

关 键 词：罗丹明B 树枝状分子 基因转染 基因载体 

分 类 号：TQ460.4[化学工程—制药化工]