毒株、苗龄及温度对烟草苗期接种TMV试验的影响  

Effects of virus strain,seedling age and temperature in TMV inoculation of tobacco at seedling stage

作　　者：申莉莉[1] 贾海燕 何青云 俞芳菲 龚明月 曲潇玲 宋丽云[1] 李莹 杨金广[1] 王凤龙[1] SHEN Li-li;JIA Hai-yan;HE Qing-yun;YU Fang-fei;GONG Ming-yue;QU Xiao-ling;SONG Li-yun;LI Ying;YANG Jin-guang;WANG Feng-long(Tobacco Research Institute of CAAS,Qingdao 266101,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101

出　　处：《植物病理学报》2021年第4期607-617,共11页Acta Phytopathologica Sinica

基　　金：农业科技创新工程项目(ASTIP-TRIC04);山东省现代农业产业技术体系项目(SDAIT-25-03);烟草绿色防控重大专项(110201601024(LS-04))。

摘　　要：病毒株系、苗龄、接种后培养条件是影响烟草病毒病发生发展的关键因素,为确保烟草接种TMV抗性鉴定和药效生测试验的准确性,在N基因烟草和普通烟草上接种TMV,采用病毒生物学、qRT-PCR和Western blot技术,研究病毒的分离纯化、株系、稀释限点、传导路径、病害症状,以及N基因抗TMV的温敏性。结果显示,TMV为U1株系,在K326上活体保存50~65 d,仍具有较强的致病力,稀释限点为10~5,接种物浓度以10~2稀释为宜。接种后TMV先由接种叶传导到主茎,向下至根、向上至心叶,然后是上部叶,最后传导至下部叶,12~16 d布满全株,21~28 d花叶畸形显著,病情调查宜在2~3周内。温度和苗龄影响N基因烟草对TMV的抗性反应,培养温度应在25℃~27℃,苗龄以6~7叶期为宜。Viral strain,seedling age and culture conditions are the key factors to influence post-inoculation induction of disease symptoms.To ensure the accuracy of tobacco’s resistance evaluation and efficacy of antiviral agents against TMV,the TMV inoculation was done on N gene and common tobacco.Assays like virus biology,qRT-PCR and Western blot,were performed to research TMV separation and purification,virus strain identification,dilution limit concentration,transmission route,symptoms development,and thermosensitivity of N gene.The results showed that TMV was U1 strain,preserved on K326 for 50-65 days still had a strong pathogenicity,with dilution limit concentration of 105.The concentration of the inoculum should be diluted by 100 times.After inoculation,TMV systemically spread from the inoculated leaf to the main stem,down to the root,up to the topmost leaf,then to the upper leaf,and finally to the lower leaf.The whole plant was covered with virus in 12-16 days,leaf mosaic and deformity was significant in 21-28 days.The systematic investigation time should be within 2-3 weeks.Temperature and seedling age affected the resistance response of N gene tobacco to TMV.The culture temperature after inoculation should be maintained at 25℃-27℃,and the seedling age should be 6-7 leaves.

关 键 词：烟草花叶病毒 传导路径 稀释限点 N基因温敏性 

分 类 号：S432.41[农业科学—植物病理学]