矮龙船花枯梢病病原菌Lasiodiplodia hormozganensis的鉴定  

Identification of the pathogen Lasiodiplodia hormozganensis causing Ixora williamsii dieback

作　　者：张绍刚 胡美姣[2] 李敏[2] 王义[1] 高兆银[2] 洪小雨 赵超[1] ZHANG Shao-gang;HU Mei-jiao;LI Min;WANG Yi;GAO Zhao-yin;HONG Xiao-yu;ZHAO Chao(School of Life and Pharmaceutical Sciences,Hainan University,Haikou 570228,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture and Rural Affairs,Haikou 571101,China)

机构地区：[1]海南大学生命科学与药学院,海口570228 [2]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室,海口571101

出　　处：《植物病理学报》2021年第4期646-649,共4页Acta Phytopathologica Sinica

基　　金：农业农村部财政专项(No.NFZX2018)。

摘　　要：矮龙船花(Ixora williamsii Sandwith)又称小叶龙船花,茜草科龙船花属,常绿灌木或小乔木,是我国亚热带重要的优良绿化树种[1,2],因其花姿优美、花色丰富、花期长而备受人们喜爱。2019年5月,调查发现海南省三沙市矮龙船花病害发生普遍,主要表现为新梢枯萎,严重时导致整株死亡。为了明确引起该病害的病原菌种类,本研究对矮龙船花枯梢病进行了病原菌的分离、致病性测定、形态学观察及分子生物学鉴定,以期为矮龙船花枯梢病有效防治提供参考依据。In May 2019, a new dieback on Ixora williamsii Sandwith was found during the disease surveys of the plants in the Sansha City, Hainan Province. The fungal pathogen YXD-918-1 was isolated by methods of tissue isolation and purified using single-spore isolation. Based on morphological characteristics and phylogenetic analysis of ITS region, EF1-α and TUB gene sequences, the fungal isolate was identified as Lasiodiplodia hormozganensis. The pathogenicity test confirmed that this species isolates were pathogen on I. williamsii. This is the first report of the dieback on I. williamsii caused by L. hormozganensis in China.

关 键 词：枯梢病 龙船花 花姿优美 病原菌种类 致病性测定 分子生物学鉴定 病害发生 绿化树种 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]