Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production  

作　　者：Vishalakshi Balija Umakanth Bangale Senguttuvel Ponnuvel Kalyani Makarand Barbadikar Srinivas Prasad Madamshetty Sanjeeva Rao Durbha Hari Yadla Sheshu Madhav Maganti 

机构地区：[1]Biotechnology Division,ICAR-Indian Institute of Rice Research,Rajendra Nagar,Hyderabad 500030,India [2]Hybrid Rice Division,ICAR-Indian Institute of Rice Research,Rajendra Nagar,Hyderabad 500030,India [3]Plant Pathology Division,ICAR-Indian Institute of Rice Research,Rajendra Nagar,Hyderabad 500030,India [4]Plant Physiology Division,ICAR-Indian Institute of Rice Research,Rajendra Nagar,Hyderabad 500030,India [5]Regional Agricultural Research Station,Professor Jayashankar Telangana State Agricultural University,Warangal 506006,India [6]Department of Biotechnology,Acharya Nagajna University,Guntur 522510,India

出　　处：《Rice science》2021年第5期493-500,I0033-I0036,共12页水稻科学（英文版）

摘　　要：Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality.However,the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast disease.The genetic improvement of Varalu was done by introgressing a major yield QTL,qDTY_(12.1),along with two major blast resistance genes i.e.Pi54 and Pi1 through marker-assisted backcross breeding.Both traits were transferred till BC_(2) generation and intercrossing was followed to pyramid the two traits.Stringent foreground selection was carried out using linked markers as well as peak markers(RM28099,RM28130,RM511 and RM28163)for the targeted QTL(qDTY_(12.1)),RM206 for Pi54 and RM224 for Pi1.Extensive background selection was done using genome-wide SSR markers.Six best lines(MSM-36,MSM-49,MSM-53,MSM-57,MSM-60 and MSM-63)having qDTY_(12.1) and two blast resistance genes in homozygous condition with recurrent parent genome of 95.0%-96.5% having minimal linkage drag of about 0.1 to 0.7 Mb were identified.These lines showed yield advantage under drought stress as well as irrigated conditions.MSM-36 showed better performance in the national coordinated trials conducted across India,which indicated that improved lines of Varalu expected to replace Varalu and may have an important role in sustaining rice production.The present study demonstrated the successful marker-assisted pyramiding strategy for introgression of genes/QTLs conferring biotic stress resistance and yield under abiotic stress in rice.

关 键 词：blast resistance drought stress marker-assisted gene pyramiding rainfed ecosystem upland rice 

分 类 号：S511[农业科学—作物学]