长链非编码RRS1-AS1/miR-142-3p/TRIM24分子轴对脂多糖诱导的血管内皮细胞凋亡的影响  

Effect of lncRNA RRS1-AS1/miR-142-3p/TRIM24 molecular axis on lipopolysaccharide-induced apoptosis of vascular endothelial cells

作　　者：李飞飞 侯欣 欧敬民[2] 张勇[2] 杨祖尉 施丽娟 LI Fei-fei;HOU Xin;OU Jing-min;ZHANG Yong;YANG Zu-wei;SHI Li-juan(Department of vascular surgery and intervention,Chongming Branch of Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 202150,China;Department of Vascular Surgery,Xinhua Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属新华医院崇明分院血管外科与介入科,上海市202150 [2]上海交通大学医学院附属新华医院血管外科

出　　处：《中国心血管病研究》2021年第8期752-756,共5页Chinese Journal of Cardiovascular Research

基　　金：国家自然科学基金(81570399)。

摘　　要：目的探讨长链非编码RNA(long non-coding RNA,lncRNA)RRS1-AS1对脂多糖诱导的血管内皮细胞凋亡的影响及可能的作用机制。方法以脂多糖10μg/ml刺激人脐静脉血管内皮细胞系HUVECs。实验设立两组:转染阴性对照质粒为对照组,转染载有RRS1-AS1序列的质粒为实验组。采用实时定量聚合酶链反应(qRT-PCR)检测转染效率。细胞增殖实验(CCK-8法)和流式细胞术分别检测两组细胞的增殖能力和凋亡情况。采用生物信息学技术预测RRS1-AS1可能的作用机制。qRT-PCR和Western blot法检测RRS1-AS1可能的靶基因表达。结果对照组和实验组HUVECs细胞中RRS1-AS1相对表达分别为(1.00±0.05)和(10.32±0.89),实验组HUVECs细胞中RRS1-AS1的表达明显增加(P<0.01),实验组细胞增殖能力明显增加(P<0.05),实验组细胞凋亡比例明显降低(P<0.01)。RRS1-AS1的靶基因可能是miR-142-3p,miR-142-3p的靶基因可能是三基序蛋白24(tripartite motif containing 24,TRIM24)。实验组和对照组HUVECs细胞miR-142-3p的表达分别为(0.32±0.08)和(1.01±0.10),实验组HUVECs细胞中miR-142-3p的表达明显减少(P<0.01),TRIM24基因的表达明显增加(P<0.01)。结论RRS1-AS1介导的miR-142-3p/TRIM24分子轴可增强脂多糖诱导的血管内皮细胞的增殖能力并抑制其凋亡。Objective To explore the effect of long non-coding RNA(lncRNA)RRS1-AS1 on lipopolysaccharide-induced vascular endothelial cell apoptosis and its possible mechanism.Methods Human umbilical vein endothelial cell line HUVECs was stimulated with lipopolysaccharide(10μg/L).Two groups were set up in the experiment:the negative control plasmid was transfected as the control group,and the plasmid with the RRS1-AS1 sequence was transfected as the experimental group.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect transfection efficiency.Cell proliferation test(CCK-8 method)and flow cytometry were used to detect the proliferation ability and apoptosis of the two groups cells.Using bioinformatics technology to predict the possible mechanism of RRS1-AS1.qRT-PCR and Western blot methods ware used to detect the possible target gene expression of RRS1-AS1.Results The relative expression of RRS1-AS1 in HUVECs cells in the control and experimental groups were(1.00±0.05)and(10.32±0.89),respectively.Compared with the control group,the expression of RRS1-AS1 in HUVECs cells in the experimental group was significantly increased(P<0.01),the cell proliferation ability of the experimental group was significantly increased(P<0.05),and the proportion of apoptosis in the experimental group was significantly reduced(P<0.01).The target gene of RRS1-AS1 might be miR-142-3p,and the target gene of miR-142-3p might be tripartite motif containing 24(TRIM24).The expression of miR-142-3p in HUVECs cells in the experimental group and control group were(0.32±0.08)and(1.01±0.10),respectively.Compared with the control group,the expression of miR-142-3p in HUVECs cells in the experimental group was significantly reduced(P<0.01)and the expression of TRIM24 gene was significantly increased(P<0.01).Conclusion RRS1-AS1 mediated miR-142-3p/TRIM24 molecular axis could enhance the proliferation and inhibit the apoptosis of vascular endothelial cells induced by lipopolysaccharide.

关 键 词：脂多糖 长链非编码RNA miR-142-3p TRIM24 凋亡 

分 类 号：Q95-33[生物学—动物学] R542.2[医药卫生—心血管疾病]