海南省野生蠓虫西藏环状病毒的研究  

A study of wild midges carrying Tibet orbivirus in Hainan province,China

作　　者：王琴燕 付士红[2] 孙定炜[3] 许秀燕 吴群 曾林海[3] 李善干[3] 何英[2] 李樊 聂凯 许松涛 殷启凯 王环宇[2] 鲁晓晴[1] 梁国栋[2] WANG Qin-yan;FU Shi-hong;SUN Ding-wei;XU Xiu-yan;WU Qun;ZENG Lin-hai;LI Shan-gan;HE Ying;LI Fan;NIE Kai;XU Song-tao;YIN Qi-kai;WANG Huan-yu;LU Xiao-qing;LIANG Guo-dong(Public Health Institute,Qingdao University,Qingdao,Shandong 266071,China;State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Hainan Center for Disease Control and Prevention,Haikou,Hainan 570203,China)

机构地区：[1]青岛大学公共卫生学院,山东青岛266071 [2]中国疾病预防控制中心病毒病预防控制所,传染病预防控制国家重点实验室,北京102206 [3]海南省疾病预防控制中心,海南海口570203

出　　处：《中国媒介生物学及控制杂志》2021年第4期415-421,共7页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(81290342);传染病预防控制国家重点实验室项目(2014SKLID103);海南省重点研发计划(ZDYF2017111)。

摘　　要：目的调查海南省琼中县吸血昆虫及其携带病毒的种类以及流行情况。方法2019年8月在当地吸血昆虫孳生环境(羊圈、猪圈)中,使用“功夫小帅”牌紫外诱蚊灯进行标本采集并使用干冰运输到实验室。采用病毒学和分子生物学方法分离病毒并对病毒分离物进行鉴定。结果共采集到蚊虫3属34只(其中库蚊26只、按蚊1只和骚扰阿蚊7只)和蠓虫(待鉴定)1450只。从采集的蠓虫标本中分离到1株可以引起白纹伊蚊C6/36细胞病变的病毒分离株(HNQZ1927),该病毒不引起金黄地鼠肾细胞(BHK-21)病变。经病毒基因扩增和核苷酸序列测定与分析,结果显示HNQZ1927为10个基因节段的环状病毒属病毒,该病毒基因编码区核苷酸(氨基酸)与环状病毒属中的西藏环状病毒(TIBOV)同源性为79.4%~98.7%(94.6%~100%),而与TIBOV以外的其他环状病毒同源性在1.2%~78.1%。病毒基因组系统进化分析表明,HNQZ1927病毒与我国此前在西藏地区采集的圆斑按蚊中分离的TIBOV XZ0906及我国在三带喙库蚊、致倦库蚊和多种蠓虫中分离的TIBOV处于共同进化分支。结论海南省蠓虫分离的HNQZ1927病毒为TIBOV,这是首次从海南省自然界采集的蠓虫标本中分离到TIBOV。Objective To investigate the species and prevalence of blood-sucking insects and their viruses in Qiongzhong county,Hainan province,China.Methods In August 2019,UV mosquito light-trapas(Gongfu Xiaoshuai)were used to collect specimens in the local environment(sheep pens and pig pens)where blood-sucking insects bred,and dry ice was used to transport the specimens to the laboratory.Virological and molecular biological methods were used to isolate viruses and identify the virus isolates.Results A total of 34 mosquitoes from 3 genera(including 26 from Culex,1 from Anopheles,and 7 Armigeres subalbatus mosquitoes)and 1450 midges(to be identified)were collected.A virus isolate(HNQZ1927)was isolated from the collected midge specimens,which could cause cytopathic effect in mosquito cells(C6/36),but did not cause cytopathic effect in BHK-21 cells.The gene amplification and nucleotide sequencing and analysis showed that HNQZ1927 was a 10-gene segment orbivirus.The nucleotides(amino acids)in the coding region of HNQZ1927 had 79.4%-98.7%(94.6%-100%)homology with those of Tibet orbivirus(TIBOV)from the genus Orbivirus,and had 1.2%-78.1%homology with those of other orbiviruses except TIBOV.The phylogenetic analysis of the viral genome showed that the HNQZ1927 virus was in the same evolutionary branch as TIBOV XZ0906,which was previously isolated from An.maculatus collected in Tibet,and the TIBOV isolated from Cx.tritaeniorhynchus,Cx.fatigans,and some species of midges in China.Conclusion The HNQZ1927 virus isolated from midges in Hainan province is TIBOV,which is the first time that TIBOV has been isolated from midge specimens collected from nature in Hainan province,China.

关 键 词：环状病毒 西藏环状病毒 蠓虫 海南省 

分 类 号：R384.5[医药卫生—医学寄生虫学] R373.3[医药卫生—基础医学]