红花香椿组培及植株再生技术研究  

Tissue Culture and Plant Regeneration of Toona rubriflora Tseng

作　　者：郑雪燕 李勇 郑天汉 彭王勋 郑宏 ZHEN Xue-yan(Fujian Yangkou State-owned Forest Farm,Shunchang,Fujian 353200)

机构地区：[1]福建省洋口国有林场,福建顺昌353200 [2]福建省林业局,福建福州353000

出　　处：《园艺与种苗》2021年第7期1-2,6,共3页Horticulture & Seed

基　　金：福建省科技厅农业引导性(重点)项目“红花香椿优良种质选育及开发利用研究”(闽科计[2018]8号)。

摘　　要：[目的]探索出一套红花香椿无性快繁技术方法。[方法]以红花香椿组织培养的方式进行无性繁殖育苗,测定不同阶段红花香椿生长情况。[结果]诱导阶段当3/4MS+6-BA0.2、NAA0.1时,新芽的比例较高,是较适宜的诱导培养基;继代阶段1/2DCR+6-BA 0.2 mg/L'+NAA 0.04 mg/L使丛生芽的增殖倍数最高;生根阶段培养基采用改良MS+IBA 1.0 mg/L+NAA 0.05 mg/L生根率最为理想;温室中炼苗时间为1周后移栽成活率较高。[结论]该研究为建立红花香椿茎段诱导及植株再生体系提供参考。[Objective]To explore a set of asexual rapid propagation technology of Toona rubriflora Tseng.[Method]The asexual propagation of Toona rubriflora Tseng was carried out by tissue culture.The growth of Toona rubriflora Tseng in different stages was determined.[Result]The results showed that,when 3/4MS+6-BA0.2、NAA0.1 were used in induction stage the proportion of new shoots was higher.It was the most suitable induction medium with the subculture stage,1/2DCR+6-BA 0.2 mg/L'+NAA 0.04 mg/L+NAA 0.04 mg/l made the multiplication multiple of clustered buds the highest.The best rooting rate was MS+IBA 1.0 mg/L+NAA 0.05 mg/L in the rooting stage medium.The survival rate of transplanting was higher after one week in greenhouse.[Conclusion]This study provided a reference for the establishment of stem segment induction and plant regeneration system of Toona rubriflora Tseng.

关 键 词：红花香椿 芽器官 离体培养 快速繁殖 

分 类 号：S792[农业科学—林木遗传育种]