The Nitrogen-Cycling Network of Bacterial Symbionts in the Sponge Spheciospongia vesparium  

作　　者：HE Liming KARLEP Liisi LI Zhiyong 

机构地区：[1]Marine Biotechnology Laboratory,State Key Laboratory of Microbial Metabolism,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [2]Joint International Research Laboratory of Metabolic&Developmental Sciences,Shanghai Jiao Tong University,Shanghai 200240,China [3]Department of Chemistry and Biotechnology,Division of Gene Technology,Tallinn University of Technology,Estonia

出　　处：《Journal of Ocean University of China》2021年第4期999-1012,共14页中国海洋大学学报（英文版）

基　　金：Financial support from the National Natural Science Foundation of China(NSFC)(Nos.31861143020,41776138)was used to conduct this research and is greatly appreciated.

摘　　要：The microbes associated with sponges play important roles in the nitrogen cycle of the coral reefs ecosystem,e.g.,nitrification,denitrification,and nitrogen fixation.However,the whole nitrogen-cycling network has remained incomplete in any individual sponge holobiont.In this study,454 pyrosequencing of the 16S rRNA genes revealed that the sponge Spheciospongia vesparium from the South China Sea has a unique bacterial community(including 12 bacterial phyla),dominated particularly by the genus Shewanella(order Alteromonadales).A total of 10 functional genes,nifH,amoA,narG,napA,nirK,norB,nosZ,ureC,nrfA,and gltB,were detected in the microbiome of the sponge S.vesparium by gene-targeted analysis,revealing an almost complete nitrogen-cycling network in this sponge.Particularly,bacterial urea utilization and the whole denitrification pathway were highlighted.MEGAN analysis suggests that Proteobacteria(e.g.,Shewanella)and Bacteroidetes(e.g.,Bizionia)are probably involved in the nitrogen cycle in the sponge S.vesparium.

关 键 词：Spheciospongia vesparium bacterial symbionts 454 pyrosequencing functional gene analysis nitrogen-cycling net-work 

分 类 号：Q938[生物学—微生物学]