乙型肝炎病毒核酸免提取荧光定量PCR方法应用价值分析  

Application Value of Quantitative PCR Method for Non-Extraction of Hepatitis B Virus Nucleic Acid

作　　者：刘丽 LIU Li(Yingkou Economic and Technological Development Zone Central Hospital(The Sixth People’s Hospital of Yingkou City),Liaoning Yingkou 115007)

机构地区：[1]营口经济技术开发区中心医院(营口市第六人民医院),辽宁营口115007

出　　处：《中国医疗器械信息》2021年第16期60-60,137,共2页China Medical Device Information

摘　　要：目的:探讨乙型肝炎病毒(HBV)核酸免提取荧光定量PCR方法应用价值。方法:选择于2017年7月~2019年7月本院收治的乙型肝炎病毒感染患者145例作为资料,均取血清提取DNA,采用ELISA(酶联免疫-吸附剂测定)法测定HBV血清标记物,采用荧光定量PCR检测血清HBV-DNA,比较两种方法检测结果。结果:ELISA法测定结果为HBeAg(+)/HBeAg(-)94例、HBeAg(-)/HBeAg(+)42例、HBeAg(-)/HBeAg(-)9例,荧光定量PCR相对应阳性率分别为100.0%、64.29%、33.33%,不同HBV血清标记物的阳性率比较差异显著,P<0.05。结论:在乙型肝炎病毒核酸检测中采用荧光定量PCR法可实现定量计数,准确反应核酸复制状况,为诊治提供可靠依据,保证治疗合理性,应用价值较高。Objective:To investigate the value of the quantitative PCR method of nucleic acid free extraction of hepatitis B virus(HBV).145 patients with hepatitis B virus infection who were admitted to our hospital from July 2017 to July 2019 were selected as data.The serum was collected to extract DNA,and HBV serum markers were determined by ELISA(enzyme-linked immunosorbent assay).Using fluorescence quantitative PCR to detect serum HBV-DNA,compare the detection results of the two methods.Results:The results of ELISA method were 94 cases of HBeAg(+)/HBeAg(-),42 cases of HBeAg(-)/HBeAg(+),9 cases of HBeAg(-)/HBeAg(-),and the corresponding positive rates of fluorescence quantitative PCR were respectively 100.0%,64.29%,33.33%,the positive rates of different HBV serum markers are significantly different,P<0.05.Conclusion:Fluorescence quantitative PCR method can be used in the detection of hepatitis B virus nucleic acid to achieve quantitative counting,accurately respond to the status of nucleic acid replication,provide a reliable basis for diagnosis and treatment,ensure the rationality of treatment,high application value.

关 键 词：乙型肝炎病毒 荧光定量PCR法 ELISA法 

分 类 号：R512.62[医药卫生—内科学]