低温胁迫下外源激素对番茄SlMYB102转录因子表达量的影响  

Effect of hormone-induced SlMYB102 transcription factor on physiological characteristics of tomato at low temperature stress

作　　者：李梦丹 王美玲 郭仰东 张喜春[1] LI Meng-dan;WANG Mei-ling;GUO Yang-dong;ZHANG Xi-chun(College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;College of Horticulture,China Agricultural University,Beijing 100193,China)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]中国农业大学园艺学院,北京100193

出　　处：《湖北农业科学》2021年第15期136-140,共5页Hubei Agricultural Sciences

基　　金：科技合作项目(2011DFR31180-3)。

摘　　要：探讨在低温胁迫下,6种外源激素对番茄转录因子SlMYB102基因表达量的影响。对长至四叶一心的番茄幼苗进行外源激素喷施和低温处理,激素处理采用水杨酸(SA)、生长素(IAA)、赤霉素(GA3)、6-苄氨基嘌呤(6-BA)、脱落酸(ABA)、茉莉酸甲酯(MeJA)进行叶面喷施,以喷施去离子水作为对照。分别在4℃低温处理后的0、1、3、6、9、12、24 h取样,利用实时荧光定量PCR法检测番茄转录因子SlMYB102基因的表达情况。结果表明,在ABA、GA3、IAA、SA、6-BA、MeJA的诱导下,SlMYB102基因对6种激素均有响应,并呈上调表达模式。SlMYB102转录因子作为MYB家族中R2R3-MYB亚家族的一员,可能参与这些激素对于逆境胁迫的调控网络。To investigate the effects of six exogenous hormones on the expression of tomato transcription factor SlMYB102 gene under low temperature stress.Exogenous hormone spraying and low temperature treatment were applied to tomato seedlings with four leaves and one heart.In hormone treatment,salicylic acid(SA),augrin(IAA),gibberellin(GA3),6-benzylamine(6-BA),abscisic acid(ABA)and methyl jasmonate(MeJA)were sprayed on the leaves.Distilled water was sprayed as a control.Sampling at 0,1,3,6,9,12 and 24 h after low temperature treatment at 4℃.The expression of tomato transcription factor SlMYB102 gene was detected by real-time fluorescence quantitative PCR.The results show that induced by ABA,GA3,IAA,SA,6-BA and MeJA,SlMYB102 gene re⁃sponded to all six hormones and presented an up-regulated expression pattern.As a member of the R2R3-MYB subfamily of MYB fam⁃ily,SlMYB102 transcription factor may be involved in the regulatory network of these hormones in response to stress.

关 键 词：番茄 低温胁迫 转录因子 实时荧光定量PCR 

分 类 号：S641.2[农业科学—蔬菜学]