青牛胆茎节组织培养关键技术研究  

Tissue culture technology of stem segments of Tinospora sagittata

作　　者：张潇月 温朝平 周宇 董凯密 陈华 王仁睿 侯大斌[1] 杨玉霞[2] 余马[1] ZHANG Xiao-yue;WEN Chao-ping;ZHOU Yu;DONG Kai-mi;CHEN Hua;WANG Ren-rui;HOU Da-bin;YANG Yu-xia;YU Ma(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China;Sichuan Academy of Traditional Chinese Medicine Sciences,Chengdu 610041,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川省中医药科学院中药材品质及创新中药研究四川省重点实验室,成都610041

出　　处：《湖北农业科学》2021年第15期144-147,共4页Hubei Agricultural Sciences

基　　金：大学生创新创业训练计划项目(S201910619033);四川省“十三五”育种攻关项目(2016NYZ0020)。

摘　　要：以青龙胆(Tinospora sagittata)的节间为研究对象,探索不同节间对腋芽诱导的影响,并针对增殖后丛生芽孱弱的情况,对组培苗壮苗培养基进行筛选。结果表明,节间位置对腋芽的诱导率和褐化率影响显著,随着节间位置靠下,诱导率逐渐下降,褐化率逐渐上升。靠近顶端的①号节间腋芽诱导率最高,为93.3%,显著高于其他位置的节间,褐化率最低,为13.3%,>0.5 cm的腋芽数量为6.7个。最佳的壮苗培养基为MS+10 mL/L EDTA,组培苗的平均苗高为4.1 cm,平均叶片数量为6.5片,叶色浓绿。Tinospora sagittate nodal segments in different positions were used as initial explants to explore the effect on axillary induc⁃tion.Media were also screened for strengthening of weak plantlets.The results showed that positions of nodal segments significantly in⁃fluenced the axillary induction and browning rate.With the positions of nodal segments from top to base of stems,the axillary induction rate decreased but browning rate increased gradually.The axillary induction rate of top no.1 nodal segment was 93.3%,which was sig⁃nificant higher than other positons of nodal segments.The browning rate of top no.1 nodal segment was 13.3%and the number of axil⁃lary buds(>0.5 cm)was 6.7.Maximum average length of shoots(4.1 cm)and average number of leaves(6.5)was recorded in the opti⁃mal strengthening media of MS+10 mL/L EDTA.

关 键 词：青龙胆(Tinospora sagittata) 节间 组织培养 壮苗培养基 

分 类 号：S567.2[农业科学—中草药栽培] Q819[农业科学—作物学]