人参枣仁颗粒改善睡眠功能的实验研究  

Study on Sleep Improving Function of Renshen Zaoren Granule(人参枣仁颗粒)

作　　者：杨铭[1] 于德伟[1] 赫慧[1] 杨明[1] YANG Ming;YU Dewei;HE Hui;YANG Ming(Jilin Academy of Chinese Medicine Sciences,Changchun,Jilin 130012)

机构地区：[1]吉林省中医药科学院,吉林长春130012

出　　处：《中国中医药科技》2021年第5期705-708,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：吉林省省级医药健康产业发展专项资金项目(20180311046YY)。

摘　　要：目的:研究人参枣仁颗粒对睡眠功能的改善作用并初步探讨其作用机制。方法:ICR小鼠随机分为4组,人参枣仁颗粒高、中、低(10.0、3.3、1.7 g/kg)剂量组和空白对照组,每日灌胃给药1次,连续30 d。观察对小鼠直接睡眠、自主活动、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验、巴比妥钠睡眠潜伏期实验、脑内γ-氨基丁酸(GABA)、5-羟色胺(5-HT)及谷氨酸(Glu)含量的影响。结果:与空白对照组比较,人参枣仁颗粒能明显减少小鼠自主活动次数(P<0.05或P<0.01),无直接睡眠作用;能延长戊巴比妥钠诱导小鼠睡眠时间(P<0.05),缩短巴比妥钠睡眠潜伏期(P<0.05或P<0.01);增加脑内GABA、5-HT含量(P<0.05或P<0.01)。结论:人参枣仁颗粒具有改善睡眠功能,其可能通过增加脑内5-HT和GABA含量发挥作用。Objective:To study the effect of Renshen Zaoren Granule on sleep function and its mechanism.Methods:ICR mice were randomly divided into 5 groups:Renshen Zaoren Granule high,medium and low(10.0,3.3,1.7 g/kg)dose group,negative control group.The test substance was given by gavage once a day for 30 days.To observe the effects of direct sleep,autonomic activity,prolonged sleep time of pentobarbital sodium,subthreshold dose hypnosis of pentobarbital sodium,sleep latency of pentobarbital sodium,GABA,5-HT and Glu contents in brain of mice were determined by ELISA.Results:Compared with the negative control group,Renshen Zaoren G ranule could significantly reduce the number of spontaneous activities in mice(P<0.05 or P<0.01),and had no direct sleep effect;it could prolong the sleep time induced by pentobarbital sodium(P<0.05),shorten the sleep latency of barbital sodium(P<0.05 or P<0.01);increase the contents of GABA and 5-HT in brain(P<0.05 or P<0.01).Conclusion:Renshen Zaoren Granule can improve sleep function,which may play a role by increasing the contents of 5-HT and GABA in brain.

关 键 词：人参枣仁颗粒 睡眠 镇静 神经递质 小鼠 

分 类 号：R285.5[医药卫生—中药学]