和肤止痒方的UPLC指纹图谱建立及8种成分的含量测定  

Establishment of UPLC Fingerprint of Hefu Zhiyang Decoction and Content Determination of 8 Components

作　　者：刘丽娟 赵亚 吴亚运 赵瑞芝 卢传坚 LIU Lijuan;ZHAO Ya;WU Yayun;ZHAO Ruizhi;LU Chuanjian(Innovative Team for Modern Interpretation of TCM Property Theory,the Second Affiliated Hospital of Guangzhou University of TCM,Guangzhou 510006,China;Dermatological Department,the Second Affiliated Hospital of Guangzhou University of TCM,Guangzhou 510120,China;Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome,Guangzhou 510120,China)

机构地区：[1]广州中医药大学第二附属医院中药药性理论现代阐释创新团队,广州510006 [2]广州中医药大学第二附属医院皮肤科,广州510120 [3]广东省中医证候临床研究重点实验室,广州510120

出　　处：《中国药房》2021年第16期1982-1987,共6页China Pharmacy

基　　金：国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)歧黄学者项目(No.国中医药办人教函〔2019〕62号);广东省省级科技计划项目(No.2017B030314166);广州市科技计划项目(No.201807010051)。

摘　　要：目的:建立和肤止痒方的指纹图谱并进行聚类分析,建立该方中8种成分的含量测定方法,为其质量控制提供参考。方法:采用超高效液相色谱法(UPLC)。以ACQUITY UPLCBEHC18为色谱柱,以乙腈-0.1%冰醋酸溶液为流动相进行梯度洗脱,流速为0.25 mL/min,柱温为30℃,检测波长为236 nm,进样量为5μL。使用《中药色谱指纹图谱相似度评价系统(2012版)》建立10批和肤止痒方的指纹图谱,与对照品比对指认色谱峰,并进行相似度评价。使用SPSS 21.0软件对10批样品进行聚类分析。采用上述UPLC法测定10批样品中没食子酸等8种成分的含量。结果:从10批和肤止痒方的UPLC指纹图谱中标定共有峰34个,指认出峰1为没食子酸、峰10为阿魏酸、峰13为落新妇苷、峰23为丹皮酚、峰28为白鲜碱、峰31为黄柏酮、峰32为梣酮、峰34为蛇床子素,与对照指纹图谱的相似度为0.923-0.979。10批样品中,S1、S2、S5、S6、S7、S8、S10聚为一类,S3、S4、S9聚为一类。上述8种成分的平均含量分别为0.596-0.714、0.262-0.321、7.647-9.859、0.113-0.644、0.170-0.202、0.854-1.281、0.631-0.857、3.243-3.548 mg/g。结论:成功建立了和肤止痒方的UPLC指纹图谱和8种成分的含量测定方法。OBJECTIVE:To establish the fingerprint and conduct cluster analysis of Hefu zhiyang decoction(HFZYD),and establish the method for content determination of 8 components,so as to provide reference for the quality control of HFZYD.METHODS:UPLC method was adopted.The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of acetonitrile-0.1%glacial acetic acid solution(gradient elution)at the flow rate of 0.25 mL/min.The column temperature maintained at 30℃,and detection wavelength was 236 nm.The sample size was 5μL.The fingerprint of 10 batches of HFZYD was established with Similarity Evaluation System of TCM Chromatographic Fingerprints(2012 edition).Compared with substance control,the common peaks were identified.The similarity evaluation was conducted.SPSS 21.0 software was used to conduct cluster analysis of 10 batches of samples.The contents of 8 components such as gallic acid in 10 batches of samples were determined by above UPLC.RESULTS:There were 34 common peaks in the UPLC fingerprint of 10 batches of HFZYD,identified as peak 1 was gallic acid,peak 10 was ferulic acid,peak 13 was astilbin,peak 23 was paeonol,peak 28 was dictamnine,peak 31 was obacunone,peak 32 was fraxinellone,and peak 34 was osthole.Its similarity with the control fingerprint was 0.923-0.979.Among 10 batches of samples,S1,S2,S5,S6,S7,S8 and S10 were clustered into one category,and S3,S4 and S9 were clustered into one category.The average contents of above 8 components were 0.596-0.714,0.262-0.321,7.647-9.859,0.113-0.644,0.170-0.202,0.854-1.281,0.631-0.857,3.243-3.548 mg/g,respectively.CONCLUSIONS:UPLC fingerprint and the method for content determination of 8 components in HFZYD are established successfully.

关 键 词：和肤止痒方 超高效液相色谱法 指纹图谱 含量测定 聚类分析 

分 类 号：R917[医药卫生—药物分析学]