盘龙七片调控Toll样受体4-骨髓样分化因子88-核因子-κB通路保护佐剂性关节炎大鼠滑膜组织  

Panlongqi tablets protect synovial tissue in rats with adjuvant arthritis by regulating Toll-like receptor 4-myeloid differentiation factor 88-nuclear factor-κB pathways

作　　者：林朋朝 周晓彬[1] 高伟静 姬艳林 冯建书[1] 李静[2] LIN Pengzhao;ZHOU Xiaobin;GAO Weijing;JI Yanlin;FENG Jianshu;LI Jing(The Third Department of Trauma,Shijiazhuang Third Hospital,Shijiazhuang,Hebei 050000,China;Department of Nursing,Hebei Geriatrics Hospital,Shijiazhuang,Hebei 050000,China)

机构地区：[1]石家庄市第三医院创伤三科,河北石家庄050000 [2]河北省老年病医院护理部,河北石家庄050000

出　　处：《安徽医药》2021年第9期1713-1717,I0001,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究盘龙七片通过调控Toll样受体4(TLR4)-骨髓样分化因子88(MyD88)-核因子-κB(NF-κB)信号通路保护类风湿关节炎(RA)大鼠滑膜组织的作用机制。方法将50只SD大鼠以随机数字表法分为正常组,模型组,低、中、高盘龙七片组,每组10只,除正常组外,其余组均采用风、寒、湿环境结合弗氏完全佐剂注射的方式制备RA模型,RA大鼠模型制备成功后,低、中、高盘龙七片组分别灌胃给予盘龙七片溶液150 mg/kg、300 mg/kg、600 mg/kg,对照组与模型组大鼠给予等量生理盐水灌胃,连续灌胃处理15 d后,切片观察滑膜组织病理形态,酶联免疫吸附测定(ELISA)检测各组大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)水平,实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测各组大鼠滑膜组织细胞中TLR4 mRNA、MyD88 mRNA以及肿瘤坏死因子受体相关因子6(TRAF-6)mRNA的表达水平,蛋白质印迹法检测滑膜组织中NF-κB p65蛋白表达情况。结果与正常组相比,模型组滑膜组织可见明显变性坏死,滑膜处可见中度充血水肿,且有大量炎细胞并伴随轻度滑膜增生,与模型组相比,不同剂量盘龙七片处理后关节变性坏死情况明显改善,滑膜组织充血水肿情况减轻,炎细胞浸润情况极大的改善,且呈浓度依赖;模型组大鼠血清中TNF-α、IL-1分别为(1.26±0.11)μg/L、(0.35±0.08)μg/L,显著高于正常组的(0.68±0.05)μg/L、(0.11±0.02)μg/L,滑膜组织中TLR4、MyD88、TRAF-6的mRNA相对表达水平分别为(2.56±0.21)、(3.83±0.26)、(2.78±0.23),显著高于正常组(1.00±0.02)、(1.01±0.02)、(1.00±0.01)(P<0.05),NF-κB p65蛋白磷酸化水平为(1.37±0.22),显著高于正常组(0.21±0.06)(P<0.05),与模型组相比,不同剂量盘龙七片组大鼠血清中TNF-α、IL-1水平显著降低(P<0.05),滑膜组织中TLR4、MyD88、TRAF-6的mRNA相对表达水平显著降低(P<0.05),NF-κB p65蛋白磷酸化水平也显著降低(P<0.05)。结论盘龙七片�Objective To study the mechanism of Panlongqi tablets on protecting synovial tissue in rats with rheumatoid arthritis(RA) by regulating Toll-like receptor 4(TLR4)-myeloid differentiation factor 88(MyD88)-nuclear factor-κB(NF-κB) signaling pathways.Methods According to random number table method, 50 SD rats were randomly assigned into normal group, model group, low-dose, medium-dose and high-dose Panlongqi tablets groups, with 10 cases in each group. Except for normal group, the other groups were given wind-cold-wet environment and Freund’s complete adjuvant injection to prepare RA models. After successful preparation of RA rat models, low-dose, medium-dose and high-dose Panlongqi tablet groups were intragastrically administered with Panlongqi tablet solutions of 150 mg/kg, 300 mg/kg and 600 mg/kg, respectively. The control group and model group were given the same amount of normal saline. After 15 d of continuous intragastric administration, slice observation was performed on pathological morphology of synovial tissue. The enzyme-linked immunosorbent assay(ELISA) was applied to detect levels of serum tumor necrosis factor α(TNF-α) and interleukin-1(IL-1) in each group. The real-time fluorescence quantitative polymerase chain reaction(q RT-PCR) was applied to detect the expression levels of TLR4 mRNA, MyD88 mRNA and TNF-α receptor associated factor 6(TRAF-6) mRNA in synovial tissue cells of each group. Western blotting was applied to detect the expression of NF-κB p65 protein in synovial tissue.Results Compared with normal group, there was significant degenerative necrosis of synovial tissue in model group. Moderate congestive edema could be found at synovium site, and there was a large number of inflammatory cells, accompanied with mild synovial hyperplasia. Compared with model group, after treatment with different doses of Panlongqi tablets, degenerative necrosis of joints was significantly improved, congestive edema of synovial tissue was alleviated, and inflammatory cells infiltration was greatly impro

关 键 词：关节炎 实验性 盘龙七片 TOLL样受体4 骨髓样分化因子88 核因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]