ELISA法检测抗HIV抗体准确度研究  

Study on the Accuracy of ELISA for Detection of Anti HIV Antibody

作　　者：陈伟[1] CHEN Wei(Leizhou Center for Disease Control and Prevention,Leizhou Guangdong,524200,China)

机构地区：[1]雷州市疾病预防控制中心,广东雷州524200

出　　处：《质量安全与检验检测》2021年第3期134-136,共3页QUALITY SAFETY INSPECTION AND TESTING

摘　　要：为探讨ELISA法检测抗HIV抗体准确度,本文选取我院检验科接受艾滋病检测的11250名受检者,采集静脉血标本,分别应用ELISA法、ECLIA法行抗HIV抗体检测。比较2种方法的检测结果、确认结果差异及确认阳性份数。结果显示ELISA检验阳性率为0.293%(33/11250),ECLIA检验阳性率为0.320%(36/11250);差距均衡(P>0.05)。ELISA检验以1.35-1.80临界数据居多,占阳性血液样本例数的56.25%(18/32),ECLIA检验以CO150-220临界数据居多,占阳性血液样本例数的59.37%(19/32)。差距均衡(P>0.05)。可见针对抗HIV抗体人群进行初筛,ELISA法与ECLIA法在筛查阳性率,检验准确度方面无显著差异,应用价值均较高,可推广。To explore the accuracy of ELISA in the detection of anti HIV antibody.11250 HIV/AIDS patients in the laboratory of our hospital were selected.Venous blood samples were collected,and anti HIV antibody was detected by ELISA and ECLIA respectively.The results of the two methods were compared.The positive rates of ELISA and ECLIA were 0.293%(33/11250)and 0.320%(36/11250);the difference was balanced(P>0.05).In ELISA test,the critical data of 1.35-1.80 accounted for 56.25%(18/32)of positive blood samples,and co150-220 critical data accounted for 59.37%(19/32)of positive blood samples in ECLIA test.The gap was balanced(P>0.05).There is no significant difference between ELISA and ECLIA in the screening positive rate and test accuracy of anti HIV antibody population screening,which has high application value and can be popularized.

关 键 词：ELISA法 抗HIV抗体 准确度 

分 类 号：R512.91[医药卫生—内科学]