小麦多酚氧化酶基因Ppo-A1和Ppo-D1位点等位变异与穗发芽抗性的关系  

Relationship between the allelic variations at the Ppo-A1 and Ppo-D1 loci and pre-harvest sprouting resistance in wheat

作　　者：黄义文 代旭冉 刘宏伟[1] 杨丽[1] 买春艳 于立强 于广军[3] 张宏军[1] 李洪杰[1] 周阳[1] HUANG Yi-Wen;DAI Xu-Ran;LIU Hong-Wei;YANG Li;MAI Chun-Yan;YU Li-Qiang;YU Guang-Jun;ZHANG Hong-Jun;LI Hong-Jie;ZHOU Yang(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Crop Molecular Breeding,Beijing 100081,China;Center for Technological Innovation of Dwarf-male-sterile Wheat Breeding(Xinxiang),Xinxiang 453731,Henan,China;Zhaoxian Experiment Station,Shijiazhuang Academy of Agricultural and Forestry Sciences,Zhaoxian 051530,Hebei,China)

机构地区：[1]中国农业科学院作物科学研究所/作物分子育种国家工程实验室,北京100081 [2]新乡县矮败小麦育种技术创新中心,河南新乡453731 [3]石家庄市农林科学研究院赵县实验基地,河北赵县051530

出　　处：《作物学报》2021年第11期2080-2090,共11页Acta Agronomica Sinica

基　　金：河北省现代种业科技专项(19226351D);国家重点研发计划项目(2017YFD0101000);中国农业科学院创新工程项目资助。

摘　　要：Ppo-A1和Ppo-D1是控制小麦多酚氧化酶(polyphenol oxidase,PPO)活性的关键基因。有报道PPO活性与穗发芽抗性有关,但Ppo-A1和Ppo-D1位点不同等位变异对穗发芽抗性的影响尚不明确。本研究利用248份我国主栽小麦品种3年发芽指数,结合Ppo-A1和Ppo-D1位点等位变异分型结果,研究两个位点不同等位变异及其等位变异组合与穗发芽抗性的关系。结果表明,发芽指数主要受年份、Ppo-A1位点和Ppo-A1×Ppo-D1互作共同影响。在Ppo-A1位点,携带低PPO活性等位变异Ppo-A1b的小麦品种发芽指数显著低于携带高PPO活性等位变异Ppo-A1a的品种,平均发芽指数相差5.22%;相反,在Ppo-D1位点携带低PPO活性等位变异Ppo-D1a品种的发芽指数高于携带高PPO活性等位变异Ppo-D1b的品种,但差异不显著。在4种等位变异组合中,Ppo-A1bPpo-D1b组合类型品种的发芽指数最低。上述Ppo-A1位点等位变异与穗发芽抗性的关系在轮选13×济麦20 F2及F2:3分离群体中得到验证。PPO活性和Ppo-A1相对表达量均与发芽指数呈显著正相关。本研究表明,Ppo-A1b等位变异的分子标记可以有效地用于穗发芽抗性辅助选择。Ppo-A1 and Ppo-D1 are the major genes that control the activity of polyphenol oxidase(PPO)in wheat.It has been reported that the activity of polyphenol oxidase affects pre-harvest sprouting(PHS)resistance,but the effect of different al-leles/allelic combinations at the Ppo-A1 and Ppo-D1 loci on PHS resistance remains unclear.The current study was carried out to elucidate the effects based on the germination index obtained from 248 Chinese wheat cultivars in a three-year trial in combina-tion with genotypic data at the Ppo-A1 and Ppo-D1 loci.Analysis of variation for the Ppo-A1 and Ppo-D1 loci showed that year,Ppo-A1 locus and Ppo-A1×Ppo-D1 interaction had significant effects on germination index.At locus Ppo-A1,germination index of cultivars carrying the allele Ppo-A1b of low PPO activity was 5.22%lower than that carrying the allele Ppo-A1a of high PPO activity on average.In contrast,the cultivars carrying the allele Ppo-D1a of low PPO activity had higher germination index than that carrying the allele Ppo-D1b of high PPO activity at locus Ppo-D1,but no significant differences between two alleles.Among the four allelic combinations,the cultivars with the Ppo-A1bPpo-D1b had the lowest germination index.The relationship between the alleles at locus Ppo-A1 and PHS resistance had been verified in the Lunxuan 13×Jimai 20 F2 and F2:3 segregation populations.There were significantly positive correlations between PPO activity/relative expression level of Ppo-A1 gene and germination index.This study suggests that functional markers of allele Ppo-A1b can be effectively applied in marker-assisted selection for PHS resistance.

关 键 词：小麦 抗穗发芽育种 多酚氧化酶 Ppo-A1b等位变异 分子标记辅助选择 

分 类 号：S512.1[农业科学—作物学]