转P_(SAG12)-IPT基因对棉花叶片衰老及产量和纤维品质的影响  

Effects of P_(SAG12)-IPT gene expression on leaf senescence,yield,and fiber quality in cotton

作　　者：王晔[1,2] 刘钊 肖爽 李芳军 吴霞 王保民 田晓莉 WANG Ye;LIU Zhao;XIAO Shuang;LI Fang-Jun;WU Xia;WANG Bao-Min;TIAN Xiao-Li(College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;College of Agronomy and Biotechnology,China Agricultural University/China Engineering Research Center of Plant Growth Regulator,Ministry of Education,Beijing 100193,China;Shanxi Agricultural University/Cotton Research Institute Shanxi Academy of Agricultural Sciences,Yuncheng 044000,Shanxi,China)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]中国农业大学农学院/植物生长调节剂教育部工程研究中心,北京100193 [3]山西农业大学/山西农业科学院棉花研究所,山西运城044000

出　　处：《作物学报》2021年第11期2111-2120,共10页Acta Agronomica Sinica

基　　金：国家转基因生物新品种培育重大专项(2011ZX08005-004-008);国家自然科学基金项目(31701337)资助。

摘　　要：探讨P_(SAG12)-IPT基因导入对棉花叶片衰老性状、产量和纤维品质的影响。本研究以9个转化事件的纯合阳性株系为试验材料,于2011—2012年在中国农业大学上庄实验站开展研究。结果表明,转基因株系叶片衰老阶段IPT基因表达量大幅上调,Z+ZR类细胞分裂素含量显著高于野生型冀合321。根据Z+ZR类细胞分裂素含量将9个转P_(SAG12)-IPT基因株系聚类分为抗衰老能力强、弱和中间型3大类,不同类型株系在叶片衰老阶段的叶绿素和可溶性蛋白含量较野生型增加幅度不同。转基因株系单株成铃数(直径≥2 cm)和铃重与野生型相当或高于野生型,但各株系第一次收获时的吐絮率显著低于野生型,且降低幅度与抗衰老能力成正比。这导致抗衰老能力较强和中等的P_(SAG12)-IPT过表达株系(OE-37和OE-38)因有效吐絮铃少而减产,而抗衰老能力弱的株系(OE-30)籽棉产量与野生型相当。P_(SAG12)-IPT基因过表达株系的纤维长度整齐度指数显著高于野生型,马克隆值有增加趋势,其他品质指标与野生型差别不大。A chimeric gene of bacterial IPT and the senescence-specific SAG12 promoter from Arabidopsis(P_(SAG12)-IPT)was overexpressed in cotton,and nine independent transgenic lines were obtained.To identify the leaf senescence characteristics,yield,and fiber quality of P_(SAG12)-IPT cotton plants,we performed the experiments at the Shangzhuang Experimental Station of China Agricultural University in 2011 and 2012.The results showed that the expression of IPT genes in transgenic lines was dramati-cally up-regulated at leaf aging stage,and the content of Z+ZR-type cytokinin was significantly higher than that of wild type Jihe 321.According to the content of Z+ZR-type cytokinin,nine transgenic lines were clustered into three categories with strong,weak,and moderate anti-aging ability.During leaf senescence period,the contents of chlorophyll and soluble proteins in transgenic lines were positively related to the content of Z+ZR-type cytokinin.Compared to wild type,transgenic lines had a higher or equal number of bolls(diameter≥2 cm)per plant and boll weigh,but their boll-opening rate at the first harvest was significantly de-creased,and the extent of reduction was positively related to the anti-aging ability.Consequently,the seed cotton yield of P_(SAG12)-IPT lines(OE-37 and OE-38)with strong or moderate anti-aging ability decreased due to less opened bolls,while the line(OE-30)with weak anti-aging ability had a comparable yield to that of wild type.The fiber length uniformity of P_(SAG12)-IPT cotton lines was significantly higher than that of wild type.Compared with wild type,the micronaire results of transgenic lines revealed an increasing trend,and fiber length,breaking strength,and breaking elongation was not significantly different from that of wild type.

关 键 词：棉花 P_(SAG12)-IPT基因 细胞分裂素 产量 纤维品质 

分 类 号：S562[农业科学—作物学]