烟草非特异性脂质转移蛋白基因家族的鉴定与分析  

Identification and analysis of non-specific lipid transfer protein family in tobacco

作　　者：李鹏 刘彻 宋皓 姚盼盼 苏沛霖 魏跃伟[1] 杨永霞[1] 李青常[2] LI Peng;LIU Che;SONG Hao;YAO Pan-Pan;SU Pei-Lin;WEI Yao-Wei;YANG Yong-Xia;LI Qing-Chang(Tobacco College,Henan Agricultural University,Zhengzhou 450002,Henan,China;Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,Henan,China)

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]中国烟草总公司郑州烟草研究院,河南郑州450001

出　　处：《作物学报》2021年第11期2184-2198,共15页Acta Agronomica Sinica

基　　金：河南省科技攻关计划(农业领域)项目(182102110315,192102110002,192102110121);河南省大学生创新创业训练计划项目(S202010466013);河南农业大学本科实验室开放创新项目(KF1908)资助

摘　　要：植物非特异性脂质转移蛋白(non-specific lipid transfer proteins, nsLTPs)可以在体外转移脂质,调节植物的生长发育以及对环境非生物和生物胁迫作出反应等。本研究从烟草栽培品种K326 (Nicotiana tabacum L.)基因组中鉴定出74个nsLTPs基因,对其系统发育关系、基因结构、保守基序、染色体定位、基因重复、启动子顺式作用元件、3D结构和激素与非生物胁迫处理下的表达模式进行了分析。结果表明,根据8个半胱氨酸之间的间隔和序列相似性将其分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅶ、Ⅷ和ⅩⅢ八种类型,相同类型的NtLTPs具有相似的内含子-外显子基因结构和保守基序模式, motif 2和motif 3是NtLTPs基因家族的特征基序。在进化过程中,片段重复是NtLTPs基因家族扩展的主要原因,干旱处理后的RNA-seq分析发现,在进化过程中,不同基因重复事件发生后功能分化模式存在差异。启动子分析表明,它们含有多种光反应、激素和非生物胁迫响应顺式作用元件。进一步采用qRT-PCR分析发现, NtLTPs家族基因在烟草植株的不同组织和器官中具有不同的表达模式,可以响应干旱、盐等非生物胁迫以及IAA、GA、SA等激素处理。研究结果为深入分析NtLTPs家族基因的功能提供了理论参考,并为进一步的分子育种提供了理论基础。Plant non-specific lipid transfer proteins(nsLTPs) can transfer lipids in vitro, regulate plant growth and development, and respond to environmental abiotic and biotic stresses. In this study, 74 nsLTPs genes were identified from the genome of Nicotiana tabacum variety K326, and we analyzed multiple characteristics of these genes, including phylogeny, gene structures, conserved motifs, protein domains, chromosome locations, cis-elements in the promoter sequences, 3 D structure, and the expression patterns under different hormones and abiotic stresses. The results revealed that nsLTPs in tobacco could be divided into eight types, including type Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅶ, Ⅷ, and ⅩⅢ, according to the interval and sequence similarity between the eight cysteines. The same types of NtLTPs had similar intron-exon patterns and conserved motifs, motif 2 and motif 3 were the characteristic motifs of Nt LTPs family. In the process of evolution, fragment duplication dominated the expansion of the NtLTPs family. RNA-seq analysis after drought treatment revealed that the functional differentiation patterns of repeat gene pairs were diverse during evolution period. Promoter analysis showed that they contained a variety of cis-acting elements in response to light response, hormones, and abiotic stress. Furthermore, qRT-PCR demonstrated that NtLTPs family genes had different expression patterns in different tissues and organs of tobacco plants, which could respond to abiotic stresses such as drought, salt, and hormone treatments(IAA, GA, and SA etc.). These results provide a theoretical reference for the in-depth analysis of the functions of NtLTPs family genes and molecular breeding.

关 键 词：烟草 nsLTPs 基因家族 生物信息学 基因表达 

分 类 号：S572[农业科学—烟草工业]