Microbial transformation for Region-selective Hydroxylation of Z-Butylidenephthalide by Aspergillus niger CGMCC 3.739  

作　　者：Jian Zou Yongheng Wang Zhengqun Zhou Rongrong He Xinsheng Yao Hao Gao 

机构地区：[1]Institute of Traditional Chinese Medicine&Natural Products,College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,Jinan University,Guangzhou 510632,China [2]Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University,Guangzhou 510632,China [3]School of Traditional Chinese Medicine,Jinan University,Guangzhou 510632,China

出　　处：《Asian Journal of Traditional Medicines》2021年第4期203-209,共7页亚洲传统医药（英文）

基　　金：This work was financially supported by grants from the National Key Research and Development Program of China(2018 YFA0903200/2018YFA0903201);the National Natural Science Foundation of China(81925037/31900284);Chang Jiang Scholars Program(Young Scholar)from the Ministry of Education of China(Hao Gao,2017);National High-level Personnel of Special Support Program(2017RA2259);Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01Y036);K.C.Wong Education Foundation(Hao Gao,2016).

摘　　要：Microbial transformation is a useful tool for structure modification of active natural products.Z-Butylidenephthalide is the main active constituent of Angelica sinensis.In the present work,a pair of new oxidized metabolites,namely(-)(11R)-(Z)-11-hydroxy-butylidenephthalide(1a)and(+)(11S)-(Z)-11-hydroxy-butylidenephthalide(1b),were obtained from microbial transformation for region-selective 11-hydroxylation of Z-butylidenephthalide by Aspergillus niger CGMCC 3.739.Their structures,including absolute configurations,were elucidated by extensive analysis of HR-ESI-MS,NMR spectra,and the modified Mosher’s method.This is the first study on the microbial transformation of Z-butylidenephthalide,and these findings offer a tool for region-selective 11-hydroxylation of Z-butylidenephthalide.

关 键 词：microbial transformation Z-butylidenephthalide Aspergillus niger CGMCC 3.739 region-selective hydroxylation 

分 类 号：Q93[生物学—微生物学]