食管宁复方中药汤剂对反流性食管炎的治疗效果:大鼠模型研究  

Therapeutic effects of compound Chinese herbal medicine,Shiguanning decoction,on reflux esophagitis:a rat model study

作　　者：段冰霞 陈宏慈[2] 徐安 萧闵[3] 田代志[3] DUAN Bing-Xia;CHEN Hong-Ci;XU An;XIAO Min;TIAN Dai-Zhi(Clinical College of Chinese Medicine,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China;Department of Geriatrics,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,China;Experimental Center of Traditional Chinese Medicine,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学中医临床学院,武汉430065 [2]湖北省中医院老年病科,武汉430061 [3]湖北中医药大学中医药实验中心,武汉430065

出　　处：《中华老年多器官疾病杂志》2021年第8期615-620,共6页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：湖北省自然科学基金面上项目(2018CFC892)。

摘　　要：目的探索食管宁复方中药汤剂对反流性食管炎的治疗效果。方法60只雄性SD大鼠,按随机数表法分为假手术组(sham组)、模型组(M组)、食管宁高剂量组(H组)、食管宁低剂量组(L组)和西药组(W组),每组12只。采用“不全幽门结扎+食管下括约肌切开术”制备反流性食管炎大鼠模型,sham组只行开关腹手术。H组、L组和W组分别给予食管宁汤剂2g/ml、食管宁汤剂1g/ml、埃索美拉唑镁肠溶片0.24mg/ml。2周后对比各组大鼠食管黏膜组织病理积分、血浆胃动素(MTL)含量、食管组织白细胞介素-17(IL-17)mRNA表达结果、肿瘤坏死因子-α(TNF-α)mRNA表达结果。采用SPSS 22.0统计软件进行数据分析。根据数据类型,组间比较分别采用方差分析或秩和检验。结果M组食管黏膜组织病理积分最高,与sham组、H组、L组、W组差异有统计学意义(P<0.05)。血浆MTL在M组含量最低,与sham组、H组、L组、W组比较差异有统计学意义(P<0.05)。M组食管组织中的IL-17mRNA和TNF-αmRNA表达结果最高,与sham组、H组、L组、W组比较差异有统计学意义(P<0.05)。结论食管宁汤剂能上调血浆MTL,进而增加食管下括约肌压力;能下调IL-17mRNA、TNF-αmRNA表达,缓解炎症反应,对反流性食管炎有治疗效果。Objective To observe the effectiveness of Shiguanning(SGN)decoction,a traditional Chinese medicine(TCM)formula,on reflux esophagitis(RE).Methods A total of 60 male SD rats were randomly divided into sham operation group(sham group),model group(M group),low-and high-dose SGN groups(1 and 2 g/ml SGN,L and H groups),and the positive control group(esomeprazole magnesium enteric-coated tablet 0.24 mg/ml,W group),each with 12 rats.The rat model of RE was established with pyloric ligation and lower esophageal sphincter incision operation.The sham group only underwent open and closed abdominal surgery.Then the histopathological score,plasma content of motilin(MTL)and mRNA levels of interleukin-17(IL-17)and tumor necrosis factor-α(TNF-α)of esophageal tissues were compared among the groups.SPSS statistics 22.0 was used to perform the statistical analysis.Chi-square test or rank sum test was employed for intergroup comparison depending on different data types.Results The M group had the highest histopathological score of esophageal mucosa,lowest plasma content of MTL,and highest mRNA levels of IL-17 and TNF-αwhen compared with the other 4 groups(all P<0.05).Conclusion SGN can elevate the plasma content of MTL,thereby increasing the pressure of the lower esophageal sphincter;inhibit the mRNA expression of IL-17 and TNF-αin esophageal tissue,thus alleviating the inflammatory response.SGN have therapeutic effects on RE.

关 键 词：食管炎 消化性 食管宁 胃动素 白介素-17 肿瘤坏死因子-Α 

分 类 号：R289.5[医药卫生—方剂学]