多凝集红细胞致患儿交叉配血次侧凝集的诊断特征分析  

Diagnostic feature analysis of secondary agglutination of cross matching in children caused by polyagglutination of red blood cells

作　　者：孔凡生 耿微[1] Kong Fansheng;Geng Wei(Blood Type Reference Laboratory, The Blood Center of Jining, Jining 272113, China)

机构地区：[1]济宁市中心血站血型参比实验室,272113

出　　处：《中华诊断学电子杂志》2021年第3期187-191,共5页Chinese Journal of Diagnostics(Electronic Edition)

摘　　要：目的探讨多凝集红细胞致患儿交叉配血次侧凝集的特点及检测方法。方法回顾性分析2019年1月至12月济宁市中心血站血型参比实验室收到的8例交叉配血次侧凝集患儿标本的检测结果。经ABO和Rh血型鉴定、直接抗人球蛋白试验、抗体筛查和交叉配血等方法,排除假凝集;采用3份正常成人ABO同型血清、3份正常成人AB型血清和花生凝集素确认多凝集红细胞。结果8例患儿红细胞直接抗人球蛋白试验阴性;抗体筛查阴性;交叉配血主侧阴性,次侧盐水法和抗人球蛋白法均为阳性;患儿红细胞与正常成人ABO同型血清、AB型血清和花生凝集素均呈凝集反应,盐水法排除缗钱状假凝集。结论多凝集红细胞与绝大多数成年人血清发生凝集反应,从而导致交叉配血次侧阳性。确认红细胞多凝集现象,有助于明确交叉配血困难的原因,避免延误患儿输血。ObjectiveTo investigate the characteristics and detection methods of secondary agglutination of cross matching in children with polyagglutination of red blood cells(RBCs).MethodsThe detection results of 8 child cases with cross matching secondary hemagglutination from January to December 2019 in Blood Group Reference Laboratory of Jining Blood Center were retrospectively analyzed.After ABO and Rh blood type identification,direct antiglobulin test(DAT),antibody screening and cross matching methods,false agglutination was excluded.Polyagglutinated RBCs were confirmed by 3 normal adults of homotypic blood serum,3 normal adult AB serum and peanut agglutinin.ResultsIn the 8 cases,the DAT of RBCs antibody screenings and the primary side of cross matching blood were negative.While the results from the secondary saline method and anti-human globulin method were positive.The RBCs of the children were agglutinated with AB serum and peanut agglutinin.All the elements showed an agglutination reaction,and the saline was used to eliminate false agglutination like rouleaux.ConclusionsThe polyagglutinated RBCs agglutinate with most adults sera,which lead to secondary positive in cross matching test.Confirming the polyagglutination of erythrocytes will help to identify the causes of cross matching difficulties and avoid delaying transfusion for child patients.

关 键 词：多凝集红细胞 花生凝集素 交叉配血 T/Tn多凝集抗原与基因 

分 类 号：R44[医药卫生—诊断学]