联合光谱和激光散斑成像的小鼠脑损伤模型在体研究  

In Vivo Research of Brain Injury Model in Mice by Combined Spectroscopy and La⁃ser Speckle Imaging

作　　者：张雅檬 宁雪 李韪韬[1] 赵月梅 张欢 钱志余[1] ZHANG Yameng;NING Xue;LI Weitao;ZHAO Yuemei;ZHANG Huan;QIAN Zhiyu(College of Automation,Nanjing University of Aeronautics and Astronautics,Nanjing 211106,China;Department of Computer Engineering,Nanjing Institute of Technology,Nanjing 211167,China)

机构地区：[1]南京航空航天大学自动化学院,南京211106 [2]南京工程学院计算机工程学院,南京211167

出　　处：《数据采集与处理》2021年第4期697-704,共8页Journal of Data Acquisition and Processing

基　　金：国家自然科学基金(61875085)资助项目;国家自然科学基金重大科研仪器研制(81727804)资助项目;南京工程学院引进人才科研启动基金(YKJ202022)资助项目。

摘　　要：血流和血氧是生物体重要的生理参数,反映了生物体的机能状态。对于起病急且变化迅速的颅脑损伤,将脑血流和血氧作为监测颅脑损伤的参数,有利于颅脑损伤的诊疗和评估。论文采用光谱分析和激光散斑成像技术构建的联合测量系统,监测脂多糖诱导小鼠颅脑损伤过程中的血流血氧,并进一步分析了高渗盐水和甘露醇两种药物治疗脑损伤效果的一致性和差异性。实验结果显示,与对照组血氧血流值(1.09±0.075,0.75±0.019)相比,脂多糖能引起小鼠的脑血流(1.36±0.080)升高和脑血氧(0.62±0.021)降低,具有显著性差异。在治疗后血氧和血流逐渐恢复,且高渗盐水治疗后的恢复更加明显。研究发现,颅脑损伤后血流和血氧呈现相反变化趋势,侧面反映了颅脑损伤后血脑屏障改变导致的血脑供养失衡,而治疗剂改善后的血流血氧情况恢复,说明血流血氧可作为疗效评估的重要参数。因此采用光谱分析技术和激光散斑成像技术对血氧和血流进行联合检测为脑损伤的实时监测和药物疗效评估提供了一种技术方案。Blood flow and blood oxygen are important physiological parameters of the organism,which reflect the functional state of the organism.For brain injury with rapid onset and pathological changes,taking cerebral blood flow and blood oxygen as parameters for monitoring head injury is conducive to the diagnosis,treatment and evaluation of head injury.The paper uses a combined measurement system constructed by spectroscopy analysis and laser speckle imaging technology to monitor the blood flow and blood oxygen in the process of lipopolysaccharide induced brain injury in mice,and further analyzes the consistency and difference of the treatment effects of brain injury with hypertonic saline and mannitol.Experimental results show that compared with the blood oxygen and blood flow value of the control group(1.09±0.075,0.75±0.019),lipopolysaccharide could increase the cerebral blood flow(1.36±0.080)and decrease the cerebral blood oxygen(0.62±0.021)in mice,which has significant difference.Blood oxygen and blood flow gradually recover after treatment,and the recovery is more obvious after hypertonic saline treatment.It is found that the blood flow and blood oxygen reveal an opposite trend after brain injury,which reflects the imbalance of blood brain maintenance caused by the change of the blood-brain barrier after brain injury.However,the blood flow and blood oxygen recover after the improvement of therapeutic agents,indicating that the blood flow and blood oxygen could be used as two important parameters for therapeutic evaluation.Therefore,the combined monitoring of blood oxygen and blood flow using spectroscopy analysis technology and laser speckle imaging technology provides a technical solution for real-time monitoring of brain injury and therapeutic evaluation.

关 键 词：脂多糖 血流血氧关联监测 实时在体光谱分析 激光散斑衬比成像 高渗盐水与甘露醇联合治疗 

分 类 号：R318[医药卫生—生物医学工程]