Fluorescein-based monitoring of RNA N^(6)-methyladenosine at single-nucleotide resolution  

作　　者：Xiao-Min Liu Shen Wang Xianqing Gan Shu-Bing Qian Jun Zhou 

机构地区：[1]School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China [2]State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,China [3]Division of Nutritional Sciences,Cornell University,Ithaca,NY 14853,USA

出　　处：《Journal of Molecular Cell Biology》2021年第4期325-328,共4页分子细胞生物学报（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(81974527);the Natural Science Foundation of Jiangsu Province,China(BK20190533);State Key Laboratory of Natural Medicines,China Pharmaceutical University(SKLNMZZRC201809)to J.Z.,from US National Institutes of Health(R01GM1222814 and R21CA227917);HHMI Faculty Scholar(55108556)to S.-B.Q.,and from China Pharmaceutical University Research Start-up Fund(3150040072)to X.-M.L.J.Z.,X.-M.L.,and S.-B.Q.conceived the project X.-M.L.and J.Z.performed most of the experiments and wrote the manuscript S.W.and X.G.helped with cell culture and data collection.All authors discussed the results and edited the manuscript.

摘　　要：Dear Editor,N^(6)-methyladenosine(m^(6)A)emerges as an abundant chemical modification on RNAs,which enriches in distinct internal regions linked to divergent aspects of RNA fate(Zhao et al.,2017).Transcriptome-wide mapping of m^(6)A sites using high-throughput sequencing enables comparative analysis of cellular^(6)A dynamics on particular RNAs under both physiological and stress conditions(Dominissini et al.,2012;Meyer et al.,2015),yet is confined to low resolution of coverage and indistinction of adjacent m6A sites.

关 键 词：al. 6 RESOLUTION 

分 类 号：Q52[生物学—生物化学]