ZMIZ1基因单核苷酸多态性与内蒙古蒙汉族寻常型银屑病相关性研究  

Relationship Between ZMIZ1 Gene Single Nucleotide Polymorphism and Psoriasis Vulgaris in Inner Mongolia

作　　者：黄艳平[1] 吕新翔[1] 孙志强[1] 李欣[1] 丁文媛 韩建文[1] Huang Yanping;LüXinxiang;Sun Zhiqiang;Li Xin;Ding Wenyuan;Han Jianwen(The Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010010,China)

机构地区：[1]内蒙古医科大学附属医院,内蒙古自治区呼和浩特010010

出　　处：《中国中西医结合皮肤性病学杂志》2021年第4期329-333,共5页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：内蒙古自治区自然科学基金项目(2018MS08030);内蒙古医科大学青年创新基金项目(YKD2018QNCX068);国家自然科学基金(81660513)。

摘　　要：目的探讨ZMIZ1基因单核苷酸多态性与内蒙古地区蒙汉族寻常型银屑病(PsV)的相关性。方法收集汉族PsV1030例,蒙古族PsV379例,正常对照汉族965例,蒙古族518例。选择位于基因区域的2个单核苷酸多态性(SNP)。rs1250559_C和rs1250546_T利用二代测序法进行基因多态性检测,HLA-C*06:02利用聚合酶链反应-序列特异性引物(PCR-SSP)进行基因分型。结果与对照组相比,rs1250559_C、rs1250546_T在汉族PsV患者中的频率差异无统计学意义(P>0.05),而在蒙古族PsV患者中的频率(分别为33.5%和48.5%)显著高于对照组(分别为27.7%和43.4%,P<0.05)。基因型分析结果显示,2个SNP与汉族PsV仍无相关性(P>0.05)。而在隐性遗传模式下,rs1250559_CC在蒙古族PsV患者中的基因型频率高于对照组(P=0.012,OR=1.88);在显性遗传模式下,rs1250546_TT+TC在蒙古族PsV患者中的基因型频率高于对照组(P=0.024,OR=1.40)。在蒙古族HLA-C*06:02阳性样本中,rs1250559_C在PsV患者中的等位基因频率高于对照组(P<0.05,OR=1.42)。而在HLA-C*06:02阴性样本中,2组间等位基因频率差异无统计学意义(P>0.05)。rs1250546_T在HLA-C*06:02阳性、阴性的样本中,等位基因频率差异无统计学意义(P>0.05)。结论ZMIZ1基因SNP与内蒙古地区蒙古族PsV可能相关,与HLA-C*06:02可能存在交互作用。Objective In order to investigate the relationship between ZMIZ1 gene single nucleotide polymorphism and psoriasis vulgaris(PsV)in Inner Mongolia.Methods A total of 1030 patients in Han nationality with PsV and 965 healthy controls were collected,meanwhile 379 patients in Mongolian with PsV and 518 healthy controls were also collected.Two single nucleotide polymorphisms(SNPs)located in the area of ZMIZ1 gene were selected.rs1250546 and rs1250559 were detected by second generation sequencing and HLA-C*06:02 was genotyped by polymerase chain reaction sequence specific primer(PCR-SSP).Results There were no significant differences in the frequencies of rs1250559_C and rs1250546_T in Han nationality with PsV(P>0.05),but the frequencies of rs1250559_C and rs1250546_T were significantly higher in Mongolian with PsV(33.5%and 48.5%respectively)than those in the healthy control group(27.7%and 43.4%respectively,P<0.05).Genotype analysis showed that there was no correlation between the two SNPs and PsV in Han nationality(P>0.05).However,the frequency of the rs1250559_CC genotype was significantly higher in Mongolian patients with PsV than in the control groups(P=0.012,OR=1.88)in a recessive mode of inheritance.The frequency of the rs1250546_TT+TC genotype was significantly higher in Mongolian patients with PsV than that in the control group(P=0.024,OR=1.40)in a dominant mode of inheritance.HLA-C*06:02 positive and negative samples were analyzed in Mongolian samples.In the HLA-C*06:02 positive samples,the allele frequency of rs1250559_C in Mongolian with PsV was significantly different from that in the healthy control group(P<0.05,OR=1.42).But in HLA-C*06:02 negative samples,there were no significant differences in allele frequency of rs1250559_C between Mongolian with PsV and the healthy control group(P>0.05).There were no significant differences in allele frequency of rs1250546_T between Mongolian with PsV and the healthy control group in the HLA-C*06:02 positive and negative samples (P>0.05). Conclusion The SNPs of rs12505

关 键 词：ZMIZ1基因 寻常型银屑病 单核苷酸多态性 关联分析 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]