二棱大麦种质资源抗氧化能力的筛选鉴定  

Screening and Identification of Antioxidant Capacity of Two Rowed Barley Resources

作　　者：王美琦 刘志萍 王金波 齐海祥 王海泽 王文迪 门秀丽 宋俊良 李建波 徐寿军[1] WANG Mei-qi;LIU Zhi-ping;WANG Jin-bo;QI Hai-xiang;WANG Hai-ze;WANG Wen-di;MEN Xiu-li;SONG Jun-liang;LI Jian-bo;XU Shou-jun(College of Agriculture,Inner Mongolia Minzu University,Tongliao 028043,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古民族大学农学院,内蒙古通辽028043 [2]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《内蒙古民族大学学报（自然科学版）》2021年第4期339-344,共6页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家自然科学基金项目(31760357,31360307);内蒙古自治区科技计划项目(2020GG0059);大麦青稞产业技术体系项目(CARS-05);内蒙古民族大学博士科研启动金(BS569)。

摘　　要：针对30份不同来源的二棱大麦材料,测定超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量等指标,利用相关性分析、方差分析、隶属函数法等方法进行抗氧化能力的综合评价.结果表明,遗传多样性指数以过氧化氢酶为最大(2.126),超氧化物歧化酶为最小(1.902);3种抗氧化酶活性及丙二醛含量在基因型间均达到极显著水平;且MDA与SOD、POD、CAT之间均呈显著或极显著相关;结合隶属函数和抗氧化性综合评价,可对30份二棱大麦品种的抗氧化能力进行排序,其中,早熟一号、97B733、杨0187、CA2-1和MACQUARIE排前5名,冈2、J axana、F6(2001-84不育×2001-29)不育×2001-29、苏U啤麦5号和2B10-4511排后5名.The comprehensive evaluation of antioxidant capacity was carried out by correlation analysis,variance analysis,membership function method and the activities of superoxide dismutase(SOD),peroxide enzyme(POD),hydrogen peroxide enzyme(CAT)and propylene dialdehyde(MDA)content were measured in 30 two rowed barley germplasm from different resources in this study.The results showed that the genetic diversity index was the largest with hydrogen peroxide enzyme(2.126)and the superoxide dismutase(1.902)was the smallest.Activities of three antioxidant enzymes and content of MDA reached a significant level among genotypes,as well as MDA was significantly or extremely significantly correlated with SOD,POD and CAT.Combined with membership function and antioxidant comprehensive evaluation,the antioxidant capacity of 30 two rowed barley varieties could be sorted for precocious No.1,97B733,Yang 0187,CA2-1 and MACQUARIE in the top five,as well as Okazaki 2,J a x a n a,F6(2001-84 sterility×2001-29)infertility×2001-29,SuU Beer Wheat 5 and 2B10-4511 in the bottom five.

关 键 词：二棱大麦种质 遗传多样性分析 隶属函数值 

分 类 号：S512.3[农业科学—作物学]