宫内急性缺血缺氧对新生小鼠肺组织降钙素基因相关肽表达的影响  

Effect of acute intrauterine ischemia and hypoxia on expression of calcitonin gene-related peptide in neonatal mice's lung tissue

作　　者：李颖[1] 王友军[1] 刘榴 张培培 Li Ying;Wang Youjun;Liu Liu;Zhang Peipei(Department of Pediatrics,The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第五附属医院儿科,450000

出　　处：《国际医药卫生导报》2021年第15期2252-2256,共5页International Medicine and Health Guidance News

基　　金：2019年度河南省高等学校指导科研项目(19B320030)。

摘　　要：目的探讨宫内急性缺血缺氧对新生小鼠肺组织降钙素基因相关肽(calcitonin gene-related peptide,CGRP)表达的影响,为临床治疗新生缺血缺氧肺损伤提供依据。方法选取孕龄21 d的Wistar大鼠7只,适应性喂养后分为3组:正常对照组(n=2)、假手术对照组(n=6)、缺血缺氧组(n=6)。结扎21 d剖腹的Wistar大鼠的一侧子宫角的血管(卵巢及宫颈两端均结扎),结扎一侧的宫内胎鼠为缺血缺氧组,另外一侧子宫角的血管不予结扎,其宫内胎鼠为假手术对照组。另外设21 d正常剖腹胎鼠为正常对照组,采用放射免疫标记法与RT-PCR观察肺组织CGRP与a-CGRP mRNA、β-CGRP mRNA的表达。结果缺血缺氧组在缺氧20 min、30 min与40 min时a-CGRP mRNA[(0.775±0.549)比(0.487±0.035),(0.886±0.045)比(0.516±0.072),(1.015±0.110)比(0.501±0.474)]、β-CGRP mRNA[(0.582±0.054)比(0.289±0.079),(0.564±0.025)比(0.349±0.009),(0.679±0.071)比(0.382±0.022),P<0.001]的表达均高于正常对照组,差异均有统计学意义(均P<0.05)。肺组织匀浆中CGRP的表达随着缺氧时间的延长而增加,在缺血缺氧20 min[(1632.170±54.963)比(1135.000±15.556)、30 min[(1745.000±61.410)比(1196.000±53.740)]、40 min[(2098.670±130.029)比(1211.500±109.602)]时的相对值均高于正常对照组,差异均有统计学意义(均P<0.05)。结论宫内缺血缺氧可以引起新生小鼠肺组织、a-CGRP、β-CGRP mRNA与CGRP的表达增加,并且随着缺血缺氧时间的延长而增加。本研究对宫内缺血缺氧新生儿急性肺损伤提供了新的治疗思路。Objective To investigate the effect of acute intrauterine ischemia and hypoxia on the expression of calcitonin gene-related peptide(CGRP)in neonatal mice's lung tissue,and to provide references for the clinical treatment of neonatal hypoxic-ischemic lung injury.Methods Seven 21 d pregnant Wistar rats were selected,and were divided into a normal control group,a sham operation control group,and a hypoxia and ischemia group.The blood vessels of one uterine horn(both ends of ovary and cervix)in the Wistar rats were ligated for 21 days;and the intrauterine fetal rats were divided into a hypoxia and ischemia group,the ones on the ligated side,and a sham operation control group,the ones on the other side.The fetal rats taking laparotomization were set as a control group.The expressions of CGRP,α-CGRP mRNA,andβ-CGRP mRNA in their lung tissue were detected by radioimmunoassay and RT-PCR.Results The levels ofα-CGRP mRNA andβ-CGRP mRNA 20,30,and 40 min after hypoxia in the hypoxia and ischemia group were higher than those in the normal control group[(0.775±0.549)vs.(0.487±0.035),(0.886±0.045)vs.(0.516±0.072),and(1.015±0.110)vs.(0.501±0.474);and(0.582±0.054)vs.(0.289±0.079),(0.564±0.025)vs.(0.349±0.009),and(0.679±0.071)and(0.382±0.022);all P<0.001].The expression of CGRP in lung homogenate increased with the prolongation of hypoxia;and the levels 20,30,and 40 min after ischemia and hypoxia were hihger than those in the normal control group[(1632.170±54.963)vs.(1135.000±15.556),(1745.000±61.410)vs.(1196.000±53.740),and(2098.670±130.029)vs.(1211.500±109.602);all P<0.05].Conclusions The expressions of CGRP,α-CGRP,andβ-CGRP mRNA in neonatal mice's lung tissue can be increased by intrauterine ischemia and hypoxia,and increase with the prolongation of hypoxia.This study provides a new treatment for neonatal acute lung injury caused by intrauterine ischemia and hypoxia.

关 键 词：缺血缺氧 降钙素原相关肽 急性肺损伤 

分 类 号：R722.12[医药卫生—儿科]