不同CaCl_(2)试剂组合对西葫芦种子低温萌发的影响  

Effects of Different Combinations of CaCl_(2) Reagents on Low-temperature Germination of Summer Squash Seeds

作　　者：王艳芳[1] 李发远 李灵芝[1] 施光安 李海平[1] 段旭锦 WANG Yanfang;LI Fayuan;LI Lingzhi;SHI Guang′an;LI Haiping;DUAN Xujin(College of Horticulture,Shanxi Agricultural University,Jinzhong,Shanxi 030801;Yungang Grottoes Institute of Research,Datong,Shanxi 037007)

机构地区：[1]山西农业大学园艺学院,山西晋中030801 [2]云冈石窟研究院,山西大同037007

出　　处：《北方园艺》2021年第15期40-45,共6页Northern Horticulture

基　　金：国家农业科技成果转化资金资助项目(2014GB2A300017);山西省科技攻关资助项目(20110311016-2)。

摘　　要：以‘春翡翠’西葫芦种子为试材,采用CaCl_(2)溶液、CaCl_(2)+脲基二琥珀酸四钠(HIDS)溶液、CaCl_(2)+黄腐酸(FA)溶液、去离子水(CK)浸种,研究不同CaCl_(2)试剂组合浸种对低温下种子萌发的影响,以期为提高西葫芦抗冷性提供参考依据。结果表明:与对照相比,添加CaCl_(2)的3个处理均可提高西葫芦种子在低温下的萌发能力,表现为CaCl_(2)+FA>CaCl_(2)+HIDS>CaCl_(2)>CK。与对照相比,CaCl_(2)+FA浸种效果最好,浸种后西葫芦种子发芽率、发芽势、发芽指数、活力指数显著提高2.48%、2.48%、1.15%、14.71%;根长、鲜质量、干质量显著增加15.12%、46.47%、13.35%;CaCl_(2)+FA溶液浸种提高了芽苗中渗透调节物质含量及抗氧化酶活性,使芽苗中脯氨酸、可溶性糖、可溶性蛋白质含量、超氧化物歧化酶、过氧化物酶活性分别增加14.52%、180.00%、31.78%、1.52%、53.53%;超氧阴离子自由基、过氧化氢含量分别降低了131.97%、35.83%。综上所述,不同CaCl_(2)试剂组合均可减轻低温对种子萌发的伤害,其中CaCl_(2)与FA组合浸种缓解低温伤害效果最佳。‘Spring Jadeite’ summer squash(Cucurbita pepo L.) seeds were used as experimental materials,the seeds were soaked with CaCl_(2) solution, CaCl_(2) + Urea disuccinate tetrasodium(HIDS) solution, CaCl_(2) + Fulvic acid solution(FA) solution and deionized water(CK) to study the effects of different CaCl_(2) reagent combination on seed germination at low temperature,in order to provide reference for improving the cold resistance of summer squash.The results showed that compared with the control,the three treatments with CaCl_(2) could improve the germination ability of summer squash seeds under low temperature,which was CaCl_(2)+FA>CaCl_(2)+HIDS>CaCl_(2)>CK.Compared with the control,the germination rate,germination potential,germination index and vigor index of summer squash seeds soaked in CaCl_(2)+FA solution increased significantly by 2.48%,2.48%,1.15% and 14.71%,while the root length,fresh weight and dry weight increased by 15.12%,46.47% and 13.35%;soaking seeds in CaCl_(2)+FA solution enhanced osmotic adjustment substances and antioxidant enzyme activity,and increased proline,soluble sugar,soluble protein contents,SOD,POD activities by 14.52%,180.00%,31.87%,1.52%,53.53%,respectively.The contents of superoxide anion radica and H2O2 decreased by 131.97% and 35.83% respectively.In conclusion,different combinations of CaCl_(2) reagents could reduce the damage of low temperature on seed germination,and the combination of CaCl_(2) and FA had the best effect.

关 键 词：CaCl_(2)试剂组合 西葫芦种子 低温 萌发 

分 类 号：S642.6[农业科学—蔬菜学]