红掌分蘖芽愈伤组织诱导与再生研究  

Study on Callus Induction and Regeneration of Anthurium andraeanum Tiller Bud

作　　者：刘良梦 刘欣 赵桃娟 陈炙[1] 李佳蔓[1] 黄振[1] LIU Liangmeng;LIU Xin;ZHAO Taojuan;CHEN Zhi;LI Jiaman;HUANG Zhen(Ecological Restoration and Conservation on Forest and Wetland Key Laboratory of Sichuan Province,Sichuan Academy of Forestry,Chengdu 610081,China)

机构地区：[1]四川省林业科学研究院,森林和湿地生态恢复与保育四川重点实验室,四川成都610081

出　　处：《四川林业科技》2021年第4期83-89,共7页Journal of Sichuan Forestry Science and Technology

基　　金：四川省科技计划项目(2020CZZX02)。

摘　　要：以红掌分蘖芽为外植体,研究了芽基部愈伤组织诱导与再生的方法。结果表明,无菌分蘖苗在MS+0.5 mg·L^(−1)6-BA+0.05 mg·L^(−1) NAA+30 g·L^(−1)蔗糖的培养基,能诱导出直径0.5~1.0 cm、质地坚硬的愈伤组织,愈伤组织在1/2MS+0.2 mg·L^(−1)6-BA+0.05 mg·L^(−1) NAA+30 g·L^(−1)蔗糖的培养基中分化出芽,平均每个愈伤组织上长6个有效芽,芽苗转入1/2MS+0.2~0.5 mg·L^(−1) IBA+20 g·L^(−1)蔗糖的培养基后,可发育为苗高3 cm以上、带有不定根和气生根的生根苗。Using anthurium andraeanum tiller bud as explant,the methods of callus induction and regeneration at bud base were studied.The results showed that the sterile tiller seedlings could induce adamant callus with a diameter of 0.5—1.0 cm and hard texture in the medium of MS+0.5 mg/L 6-BA+0.05 mg/L NAA+30 g/L sucrose.The callus differentiated into buds in the medium of 1/2MS+0.2 mg/L 6-BA+0.05 mg/L NAA+30 g/L sucrose.On average,each callus had 6 effective buds.When the buds were transferred to the medium of 1/2MS+0.2—0.5 mg/L IBA+20 g/L sucrose,they could develop into rooting seedlings with a height of 3 cm or more,with adventitious roots and aerial roots.

关 键 词：红掌 分蘖 组织培养 愈伤组织 不定芽 

分 类 号：S682.14[农业科学—观赏园艺]