单操作者大鼠腹腔异位心脏移植模型的建立与改良  

Establishment and Improvement of Abdominal Heterotopic Heart Transplantation Model in Rats by Single Operator

作　　者：曾顺 刘达兴 ZENG Shun;LIU Daxing(Department of Cardiovascular Surgery,Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学附属医院心血管外科,遵义563000

出　　处：《实验动物科学》2021年第3期70-74,共5页Laboratory Animal Science

基　　金：遵市科合社字(2014)93号。

摘　　要：目的学习并改良大鼠腹腔异位心脏移植模型的建立过程,降低手术难度,减短学习周期,使模型易建立以供于移植免疫、病理、药理等方面的研究。方法建立大鼠腹部异位心脏移植模型,归纳总结整个过程中的操作技巧。结果正式实验30例,存活29例,存活率96.7%。术中刺伤心脏,开放后无法止血死亡1例,供心阻断时间为(24.2±5.0)min,手术时间为(59.8±10.0)min。结论单操作者大鼠腹腔异位心脏移植模型的操作方法经过改良后,缩短了手术时间,降低了手术难度,提高了手术成功率。Objective To study and improve the establishment process of rat abdominal heterotopic heart transplantation model,reduce the difficulty of operation,shorten the learning cycle,and make the model easy to establish for the study of transplantation immunity,pathology and pharmacology.Method Through the establishment of rat abdominal heterotopic heart transplantation model,the operation skills were summarized in the whole process.Result There were 30 cases in the formal experiment,and the survival rate was 96.7%.One case died of stabbing the heart during the operation and failed to stop bleeding after opening.The average cold ischemia time was(24.2±5.0)minutes and total operation time was(59.8±0.0)minutes.Conclusion The modified operation method of rat abdominal heterotopic heart transplantation model can shorten the operation time,reduce the difficulty of operation,and improves the sucess rate of the operation.

关 键 词：心脏移植 单操作者 异位移植 动物模型 

分 类 号：Q95-336[生物学—动物学]