液相色谱串联质谱法快速测定鸡肉和鸡肝中氯苯胍的残留量  

Fast Determination of Robenidine Residues in Chicken Meat and Livers by Liquid Chromatography-Tandem Mass Spectrometry(LC-MS/MS)

作　　者：陈心怡 李丽苏 叶智莲 刘佳娜 黄任东 赵凤娟 CHEN Xin-Yi;LI Li-Su;YE Zhi-Lian;LIU Jia-Na;HUANG Ren-Dong;ZHAO Feng-Juan(Food Inspection and Quarantine Technology Center of Shenzhen Customs,Shenzhen 518045;Shenzhen Key Laboratory of Detection Technology R&D on Food Safety,Shenzhen 518045)

机构地区：[1]深圳海关食品检验检疫技术中心,深圳518045 [2]深圳市食品安全检测技术研发重点实验室,深圳518045

出　　处：《中国口岸科学技术》2021年第7期36-42,共7页China Port Science and Technology

基　　金：国家重点研发计划项目(2017YFC1601603)。

摘　　要：建立液相色谱串联质谱法快速测定鸡肉和鸡肝中氯苯胍残留量。样品经过前处理条件优化,确定了最优条件:采用2%甲酸乙腈提取,正己烷脱脂净化,净化液经水稀释后过0.22μm尼龙滤膜。质谱检测采用正离子多反应监测模式测定,外标法定量。结果表明在1.00~20.0μg/L浓度范围内氯苯胍有良好的线性关系(r> 0.99)。方法的检出限为5.00μg/kg,定量限为10.0μg/kg,平均加标回收率为71.9%~110%,相对标准偏差在1.58%~8.68%之间。该方法操作快速、简便、准确,适用于鸡肉和鸡肝中氯苯胍残留量的快速测定。A rapid liquid chromatography-tandem mass spectrometry(LC-MS/MS) method is established for determination of robenidine residues in chicken meat and livers. The sample was optimized through pretreatment conditions, and the optimal conditions were determined that: extraction with 2% formic acid-acetonitrile, de-esterification and purification with n-hexane, and the purified solution diluted with water and filtered through 0.22 μm nylon membranes. In mass spectrometry detection, positive-ion multi-reaction monitoring mode and external standard method for quantification were employed. The results showed that robenidine had a good linear coefficient(r > 0.99) in the concentration range of 1.00-20.0 μg/L. The limits of detection and quantitation using this method were 5.00 μg/kg and 10.0 μg/kg respectively. The average recoveries and relative standard deviations(RSD) were between 71.9%-110% and 1.58%-8.68% respectively. This method proved to be simple, rapid, accurate and suitable for the rapid determination of robenidine residues in chicken meat and livers.

关 键 词：氯苯胍 残留 液相色谱-串联质谱 鸡肉和鸡肝 

分 类 号：O657.63[理学—分析化学] TS251.7[理学—化学]