唐古特大黄总蒽醌对高脂大鼠降血脂及抗动脉粥样硬化作用研究  

Study on anti-atherosclerosis and hypolipidemic effect of total anthraquinones extracts of rheum tanguticu in hyperlipemia rats

作　　者：杨昊虹 宋更选 王慧琳 孙志霞 王琳璇 谢惠春[1] YANG Hao-hong;SONG Geng-xuan;WANG Hui-lin;SUN Zhi-xia;WANG Lin-xuan;XIE Hui-chun(Qinghai Provincial Key Laboratory of Medicinal Plant and Animal Resources of Qinghai-Tibet Plateau,Xining 810008,China)

机构地区：[1]青海师范大学青藏高原药用动植物资源青海省重点实验室,青海西宁810008

出　　处：《青海师范大学学报（自然科学版）》2021年第2期49-56,共8页Journal of Qinghai Normal University(Natural Science Edition)

基　　金：青海省科技厅基础研究类项目(2017-ZJ-774);青海省科技厅项目(2020-ZJ-Y40);2018年度“西部之光”人才培养引进计划;青海省科技厅科技成果转化专项(2018-NK-131)。

摘　　要：目的:研究唐古特大黄总蒽醌对高脂大鼠血脂以及动脉粥样硬化(AS)的作用.方法:将高脂大鼠随机分为大黄总蒽醌提取物高、中、低剂量、模型组和阳性对照组,灌胃处理后分别测定各组大鼠血脂及生化指标;取肝脏称重,计算脏器指数;HE染色观察肝脏、胸主动脉形态学变化.结果:与模型组相较,大黄总蒽醌给药组均能降低TC、TG、LDL-C并升高HDL-C含量.大黄总蒽醌各剂量组ALT、AST、ALP随着蒽醌剂量的增加呈下降趋势,其中大黄总蒽醌高剂量效果优于阳性组.除此之外,大黄总蒽醌可显著减少肝脏中的炎性和细胞变性,减轻肝细胞的受损程度,并降低血钙浓度.同时,大黄总蒽醌能够减缓大鼠AS的症状,改善主动脉病变程度.结论:唐古特大黄总蒽醌可以通过改善血脂以及生化指标、炎症反应进而干预大鼠AS的形成,且存在量—效关系.Objective:To study the effect of total anthraquinones from Rheum tanguticum on blood fat and atherosclerosis(AS)in hyperlipidemia rats.Method:The hyperlipemia SD rats were divided into the model group,positive control group,total anthraquinones from Rheum tanguticum low,medium and high dose groups.After administration,the levels of lipid indexes and biochemical indexs in each group were detected.Liver coefficient were measured after weighing,then we observed the morphologicachanges in aortas and livers by using hematoxylin-eosin(HE)staining.Result:Compared with the model group,the total anthraquinones from Rheum tanguticum significantly reduced the serum levels of total cholesterol(TC),triglyceride(TG),and low density lipoprotein cholesterol(LDL-C),and significantly increased serum high density lipoprotein cholesterol(HDL-C)level.Meanwhile,with the increase of the doses,the ALT,AST,ALP of total anthraquinones groups decreased respectively,especially the high dose group was better than the positive control group.In addition,total anthraquinones improved the morphology of liver cells,significantly reduced the number of inflammatory foci and cell degeneration in the liver and reduced blood-calcium.The total anthraquinones also showed significant effect on reducing aorta lesion in rats.Conclusion Total Anthraquinones from Rheum tanguticum can protect the aorta of hyperlipidemia rats by regulating lipid metabolism,serum biochemistry indexs and inflammation in the liver and present a dose-effect relationship.

关 键 词：唐古特大黄 总蒽醌 血脂 动脉粥样硬化 

分 类 号：R917[医药卫生—药物分析学]