UPLC-Q-Exactive Orbitrap高分辨质谱定量分析不同产地、炮制前后女贞子中9种成分的含量  

UPLC-Q Exactive-Orbitrap high-resolution mass spectrometry quantitative analysis of the content of nine components in Ligustri Lucidi Fructus from different producing areas and before and after processing

作　　者：胡雁萍 赵迪[1,2,3] 李焕茹 冯志毅 李柯[1,2,3] 冯素香 HU Yan-ping;ZHAO Di;LI Huan-ru;FENG Zhi-yi;LI Ke;FENG Su-xiang(Henan University of Chinese Medicine;Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of P.R.;Zhengzhou Key Laboratory of Chinese Medicine for Quality Control and Evaluation,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学 [2]呼吸疾病中医药防治省部共建协同创新中心 [3]郑州市中药质量控制与评价重点实验室,郑州450046

出　　处：《天然产物研究与开发》2021年第8期1308-1319,共12页Natural Product Research and Development

基　　金：河南省科技攻关项目(142102310280)。

摘　　要：采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UPLC-Q-Exactive Orbitrap MS)建立了同时测定女贞子中红景天苷、芸香苷、木犀草苷、特女贞苷、橄榄苦苷、木犀草素、芹菜素、槲皮素、松果菊苷等9个成分含量的方法,对比分析不同产地、酒炖前后女贞子含量的差异。采用Hypersll GOLD C 18色谱柱(100 mm×2.1 mm,3μm),流动相为甲醇-0.1%甲酸水梯度洗脱,电喷雾离子源(ESI),负离子模式扫描,结合化学模式识别分析不同产地、炮制前后的女贞子中9个成分的变化。结果表明9个成分在各自浓度范围内线性关系良好(r≥0.9996),平均加样回收率为97.4%~101.9%。不同产地、炮制前后的女贞子中9个成分含量有明显差异,其中特女贞苷、红景天苷、木犀草苷、木犀草素、芸香苷、橄榄苦苷、松果菊苷对产地划分的贡献程度较大;女贞子经炮制后红景天苷、特女贞苷、橄榄苦苷的含量明显增加。本研究所建立的方法快速、准确、灵敏度高、重复性好,可为女贞子饮片和炮制品的质量标准完善提供科学依据。UPLC-Q-Exactive Orbitrap MS was used to establish the determination of salidroside,rutin,luteolin-7-O-glucoside,specnuezhenide,oleuropein,luteolin,apigenin,quercetin and echinacoside in Ligustri Lucidi Fructus simultaneously to compare and analyze the differences in the content of Ligustri Lucidi Fructus from different producing areas and before and after wine stewing.The Hypersll GOLD C 18 reverse phase chromatography column(100 mm×2.1 mm,3μm)was used with a gradient elution of 0.1%formic acid solution and acetonitrile with the condition of electrospray ionization and negative ion mode.Combining chemical pattern recognition to analyze nine components changes of Ligustri Lucidi Fructus from different origins and before and after processing.The nine components have a good linear relationship within their respective concentration ranges(r≥0.9996),and the average sample recovery rate is 97.4%-101.9%.The contents of nine components in Ligustri Lucidi Fructus from different habitats before and after processing are obviously different,of which specnuezhenide,salidroside,luteolin-7-O-glucoside,luteolin,rutin,oleuropein and echinacoside contribute greatly to the division of habitats;After processing,the contents of salidroside,specnuezhenide,oleuropein in Ligustri Lucidi Fructus increased significantly.The method is fast,accurate,highly sensitive,reproducible,and can provide a scientific basis for the perfect quality standards of Ligustri Lucidi Fructus and processed products.

关 键 词：女贞子 四极杆-静电场轨道阱高分辨质谱 不同产地 炮制 含量测定 

分 类 号：R282.4[医药卫生—中药学]