丝穗金粟兰化学成分及其神经保护活性研究  

Chemical constituents from Chloranthus fortunei and their neuroprotective effects

作　　者：陈芳有 陈志超 黄伟明 卞玉婷 双鹏程 李宏焘 罗永明[1] CHEN Fang-you;CHEN Zhi-chao;HUANG Wei-ming;BIAN Yu-ting;SHUANG Peng-cheng;LI Hong-tao;LUO Yong-ming(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学药学院,南昌330004

出　　处：《天然产物研究与开发》2021年第8期1333-1338,共6页Natural Product Research and Development

基　　金：国家自然科学基金(82060697,21262019);江西中医药大学博士科研启动基金(2018BSZR003)。

摘　　要：丝穗金粟兰95%乙醇提取物通过采用多种色谱技术和波谱学方法获得并鉴定出14个化合物,分别为curzerenone(1)、蓬莪术环氧酮(2)、curcodione(3)、chlorantene C(4)、(1 E,4 Z)-8-hydroxy-6-oxogermacra-1(10),4,7(11)-trieno-12,8-lactone(5)、zederone epoxide(6)、13-epitorulosol(7)、vomifoliol(8)、(-)-loliolide(9)、7,4′-dimethylnaringenin(10)、槲皮素-3-O-α-L-鼠李吡喃糖苷(11)、槲皮素-3-O-β-D-吡喃葡萄糖苷(12)、儿茶素(13)、香豆酸(14)。其中化合物1、3、8~14为首次从金粟兰属中分离得到,化合物2、4~7为首次从该植物中分离得到。在10μM浓度下,化合物1~6使H 2O 2损伤的PC12细胞的细胞存活率从43.41%±1.59%分别提高到62.61%±5.23%、64.87%±8.42%、56.06%±6.65%、65.87%±5.34%、60.54%±3.32%和68.11%±4.76%。Chloranthus fortunei(A.Gray)Solms-Laub,commonly known as“sikuaiwa”in Chinese,has been used to alleviate the sufferings of carbuncles,bruises,furuncles,and dysmenorrhea.Previous phytochemical investigations on this plant have revealed the presence of sesquiterpenoids,sesquiterpenoid dimers,diterpenoids,flavonoids,coumarins,and phenolic acids.Herein,various chromatographic techniques and spectroscopic methods were used to conduct the chemical study on the whole plants of C.fortunei.14 compounds were obtained from the 95%ethanol extract of C.fortunei and identified as curzerenone(1),zederone(2),curcodione(3),chlorantene C(4),(1 E,4 Z)-8-hydroxy-6-oxogermacra-1(10),4,7(11)-trieno-12,8-lactone(5),zederone epoxide(6),13-epitorulosol(7),vomifoliol(8),(-)loliolide(9),7,4′-dimethylnaringenin(10),quercetin-3-O-α-L-rhamnopyranoside(11),quercetin-3-O-β-D-glucopyranoside(12),catechin(13),and p-coumaric acid(14).Compounds 1,3,and 8-14 were firstly isolated from the genus Chloranthus,and compounds 2 and 4-7 were firstly obtained from this plant.Moreover,some of the isolated compounds were screened for their neuroprotective activities using CCK8 method,compounds 1-6 exhibited moderate neuroprotective effects.

关 键 词：丝穗金粟兰 curzerenone 蓬莪术环氧酮 神经保护活性 

分 类 号：R284.2[医药卫生—中药学]