双氢青蒿素对恶性胸腔积液肺癌细胞的影响  

Effect of Dihydroartemisinin on Malignant Pleural Effusion Lung Cancer Cells

作　　者：王平平 孟宇婷 李秋月 芦小单 张维杰[1,2] WANG Ping-ping;MENG Yu-ting;LI Qiu-yue;LU Xiao-dan;ZHANG Wei-jie(Changchun University of Chinese Medicine,Changchun 130117,China;Jilin Provincial People’s Hospital,Changchun 130021,China)

机构地区：[1]长春中医药大学,吉林长春130117 [2]吉林省人民医院,吉林长春130021

出　　处：《长春师范大学学报》2021年第8期77-80,共4页Journal of Changchun Normal University

基　　金：吉林省卫健委项目“胸水淋巴细胞亚群分析在胸腔积液中的应用研究”(2020J116)。

摘　　要：为探讨双氢青蒿素对恶性胸腔积液肺癌细胞的影响,分离培养了原代恶性胸腔积液肺癌细胞,采用MTT方法确定双氢青蒿素用药剂量及作用时间,荧光定量聚合酶链式反应(RT-PCR)检测Bcl2、Bax、Keap1、Nrf2等基因mRNA表达;Western Blot检测相关蛋白表达。结果显示,与对照组相比,实验组Bcl2、Keap1基因mRNA水平显著降低,Bcl2蛋白水平显著降低,Bax蛋白水平显著升高。双氢青蒿素影响恶性胸腔积液肺癌细胞的生长,其机制可能与下调Bcl2表达,增强细胞凋亡路径以及降低细胞面对氧化损伤的能力有关。To investigate the effect of dihydroartemisinin on malignant pleural effusion lung cancer cells,primary malignant pleural effusion lung cancer cells were isolated and cultured.MTT method was used to determine the dose and action time of dihydroartemisinin,and fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the mRNA expression of Bcl2,Bax,Keap1 and Nrf2 genes.The expression of related proteins was detected by Western Blot.The results showed that the mRNA levels of Bcl2 and Keap1 genes in the experimental group were significantly decreased,the protein levels of Bcl2 were significantly decreased,and the protein levels of Bax were significantly increased compared with the control group.Therefore,we believe that dihydroartemisinin affects the growth of malignant pleural effusion lung cancer cells,and the mechanism may be related to the down-regulation of Bcl2 expression,enhancement of apoptosis pathway and reduction of the ability of cells to face oxidative damage.

关 键 词：双氢青蒿素 恶性胸腔积液 肺癌细胞 细胞存活率 

分 类 号：R563.1[医药卫生—呼吸系统]