HPLC测定花椒挥发油中芳樟醇的含量  

Determination of Linalool in Volatile Oil of Pericarpium Zanthoxyli by HPLC

作　　者：王琳 易权利 何兵 Wang Lin;Yi Quanli;He Bing(School of Pharmaceutical Sciences of Southwest Medical University,Luzhou 646000;Taiji Group Chongqing Fuling Pharmaceuticals Co.Ltd.,Chongqing 408000,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]太极集团重庆涪陵制药厂有限公司,重庆408000

出　　处：《广东化工》2021年第13期204-205,共2页Guangdong Chemical Industry

基　　金：泸州市重点科技项目(2010-S-13),泸州市科技局-泸州医学院联合基金项目(2013LZLY-K61)。

摘　　要：目的:建立HPLC测定花椒挥发油中芳樟醇含量的方法。方法:色谱柱:Dikma Diamonsil C18柱(250 mm×4.6 mm,5μm);流动相:乙腈–水,梯度洗脱;检测波长205 nm;柱温30℃;流速1 mL/min。结果:芳樟醇在0.7815~6.2520μg范围内具有良好的线性关系(r=0.9999),平均回收率为99.18%,RSD为0.52%(n=9)。结论:采用HPLC测定花椒挥发油中芳樟醇的含量准确可靠,简单易行,该方法可用于花椒挥发油中芳樟醇的质量控制。Objective: To determine the content of linalool in volatile oil of Pericarpium zanthoxyli by HPLC. Methods: The separation was implemented on a Dikma Diamonsil C18 column(250 mm×4.6 mm, 5 μm). Acetonitrile-water was as mobile phase, gradient elution. The detection wavelength was 205 nm. The column temperature was 30 ℃. The flow rate was 1 m L/min. Results: The calibration curve showed good linearity over the range of 0.7815~6.2520 μg(r=0.9999).The average recovery was 99.18 % with RSD 0.52 %. Conclusion: This HPLC method is accurate and simple, which can be used to determine the content of linalool in volatile oil of Pericarpium zanthoxyli.

关 键 词：花椒 挥发油 芳樟醇 HPLC 含量测定 

分 类 号：TQ[化学工程]