Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals  

作　　者：Xin Zhang Chuan-Chuan He Jin-Ming Liu Hao Li Ke Lu Zhi-Qiang Fu Chuan-Gang Zhu Yi-Ping Liu Lai-Bao Tong De-bao Zhou Li Zha Yang Hong Ya-Mei Jin Jiao-Jiao Lin 

机构地区：[1]Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Parasitology,Ministry of Agriculture,Shanghai 200241,People’s Republic of China [2]Anhui Center for Animal Disease Control and Prevention,Hefei,People’s Republic of China [3]Wangjiang county Center for Animal Husbandry and Veterinary Bureau,Anqing,People’s Republic of China [4]Dongzhi county Center for Animal Husbandry and Veterinary Bureau,Chizhou,People’s Republic of China [5]Huangshan Center for Animal Disease Control and Prevention,Huangshan 341000,People’s Republic of China [6]Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonosea,Yangzhou,Jiangsu province 225009,People’s Republic of China

出　　处：《Infectious Diseases of Poverty》2017年第1期754-760,共7页贫困所致传染病（英文）

基　　金：This study was supported by research funds provided by the Special Fund for Agro-scientific Research in the Public Interest(grant No.201303037);the National Natural Science Foundation of China(grant No.31572218);the Scientific and Technological Innovation Program of the Chinese Academy of Agricultural Sciences。

摘　　要：Background:Schistosomiasis japonica is a common zoonosis.Domestic animals are the primary source of infection and play an important role in disease transmission.The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and,for this reason,diagnostics with a higher sensitivity have become increasingly necessary.It was reported that polymerase chain reaction(PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and specificity.The present study aimed to develop a PCR-based method for detection of Schistosoma japonicum infection in domestic animals.Methods:A specific nested-PCR assay was developed to detect S.japonicum infection in domestic animals via amplification of a 231-bp DNA fragment of retrotransposon SjR2.The developed assay was first used in sera and dry blood filter paper(DBFP)from goats and buffaloes at different time points of infection.Then,78 DBFPs from 39 artificially-infected bovines at 14 and 28 days post-infection and 42 DBFPs from schistosome-negative bovines from the city of Huangshan in the Anhui province were used to evaluate the diagnostic validity.Furthermore,this assay was used to detect S.japonicum infection in domestic animals in Dongzhi and Wangjiang counties.Results:The expected PCR product was detected in eggs and adult worms of S.japonicum and blood samples from S.japonicum-infected goats and water buffaloes,but not from Fasciola and Haemonchus contortus worms.The nested-PCR assay could detect the target S.japonicum DNA in DBFPs from goats and buffaloes after day 3 post-infection.The sensitivity in buffaloes at 14 and 28 days post-infection was 92.30%(36/39)and 100%(39/39),respectively.The specificity was 97.60%(41/42).The positivity rates in Dongzhi and Wangjiang counties were 6.00%and 8.00%in bovines and 22.00%and 16.67%in goats,respectively.The positivity rates in goats in both counties were higher than those in bovines with a significant difference in Dongzhi County but

关 键 词：SCHISTOSOMIASIS Domestic animals NESTED-PCR DIAGNOSIS 

分 类 号：F42[经济管理—产业经济]