Multi-locus variable-number tandem repeat analysis of Chinese Brucella strains isolated from 1953 to 2013  

作　　者：Guo-Zhong Tian Bu-Yun Cui Dong-Ri Piao Hong-Yan Zhao Lan-Yu Li Xi Liu Pei Xiao Zhong-Zhi Zhao Li-Qing Xu Hai Jiang Zhen-Jun Li 

机构地区：[1]State Key Laboratory for Infectious Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Prevention and Control,Beijing 102206,China [2]National Institute of Occupational Health and Poison Control,Chinese Center for Disease Prevention and Control,Beijing 100050,China [3]Department of Brucellosis Prevention and Control,Qinghai Institute for Endemic Disease Prevention and Control,Xining 811602,China

出　　处：《Infectious Diseases of Poverty》2017年第1期778-785,共8页贫困所致传染病（英文）

基　　金：This study was supported by grants from the Chinese National Programs for High Technology Research and Development:Lab Infectious Materials and Biological Risk Source Key Technology and Product Research(No.2014AA021404);the National Nature Science Foundation(No.81271900).

摘　　要：Background:Brucellosis was a common human and livestock disease caused by Brucella strains,the category B priority pathogens by the US Center for Disease Control(CDC).Identified as a priority disease in human and livestock populations,the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still lacking.Methods:A total of 600 Brucella isolates collected during 60 years(from 1953 to 2013)in China were genotyped by multiple locus variable-number tandem repeat analysis(MLVA)and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index(HGDI)values.The charts and map were processed by Excel 2013,and cluster analysis and epidemiological distribution was performed using BioNumerics(version 5.1).Results:The 600 representative Brucella isolates fell into 104 genotypes with 58 singleton genotypes by the MLVA11 assay,including B.melitensis biovars 2 and 3(five main genotypes),B.abortus biovars 1 and 3(two main genotypes),B.suis biovars 1 and 3(three main genotypes),and B.canis(two main genotypes)respectively.While most B.suis biovar 1 and biovar 3 were respectively found in northern provinces and southern provinces,B.melitensis and B.abortus strains were dominant in China.Canine Brucellosis was only found in animals without any human cases reported.Eight Brucellosis epidemic peaks emerged during the 60 years between 1953 and 2013:1955-1959,1962-1969,1971-1975,1977-1983,1985-1989,1992-1997,2000-2008 and 2010-2013 in China.Conclusions:Brucellosis has its unique molecular epidemiological patterns with specific spatial and temporal distribution according to MLVA.Trial registration:IDOP-D-16-00101.

关 键 词：BRUCELLA MLVA Molecular epidemiology 

分 类 号：R51[医药卫生—内科学]